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Science 20 April 1984:
Vol. 224. no. 4646, pp. 285 - 289
DOI: 10.1126/science.6324342
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Articles

Science, Vol 224, Issue 4646, 285-289
Copyright © 1984 by American Association for the Advancement of Science

articles

Primary structure of v-raf: relatedness to the src family of oncogenes

GE Mark and UR Rapp

A replication-defective, acute transforming retrovirus (murine sarcoma virus 3611) was isolated from mouse and molecularly cloned. The nucleotide sequence of 1.5 kilobases encompassing the transforming gene (v-raf) was determined. This sequence, which predicts the amino acid sequence of a gag-raf fusion protein, terminates 180 nucleotides from the 3' end of the acquired cellular sequence. Comparison of the predicted amino acid sequence of v-raf with the predicted amino acid sequences of other oncogenes reveals significant homologies to the src family of oncogenes. There is a lack of homology within the sequence of the tyrosine acceptor domain described for the phosphotyrosine kinase members of the src family of transforming proteins. Phylogenetic arrangement of this family of oncogenes suggests that tyrosine-specific phosphorylation may be a recently acquired activity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Immunoprecipitation of insulin receptors from cultured human lymphocytes (IM-9 cells) by antibodies to pp60src.
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Autoimmunity and increased c-myb transcription.
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