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Science 13 April 1984:
Vol. 224. no. 4645, pp. 159 - 161
DOI: 10.1126/science.6322309
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Articles

Science, Vol 224, Issue 4645, 159-161
Copyright © 1984 by American Association for the Advancement of Science

articles

Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells

E Gonczol, PW Andrews, and SA Plotkin

To study the mode of action of human cytomegalovirus, an important teratogenic agent in human populations, the susceptibility of a pluripotent human embryonal carcinoma cell line to the virus was investigated. Viral antigens were not expressed nor was infectious virus produced by human embryonal carcinoma cells after infection, although the virus was able to penetrate these cells. In contrast, retinoic acid-induced differentiated derivatives of embryonal carcinoma cells were permissive for antigen expression and infectious virus production. Replication of human cytomegalovirus in human teratocarcinoma cells may therefore depend on cellular functions associated with differentiation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Breaking Human Cytomegalovirus Major Immediate-Early Gene Silence by Vasoactive Intestinal Peptide Stimulation of the Protein Kinase A-CREB-TORC2 Signaling Cascade in Human Pluripotent Embryonal NTera2 Cells.
J. Yuan, X. Liu, A. W. Wu, P. W. McGonagill, M. J. Keller, C. S. Galle, and J. L. Meier (2009)
J. Virol. 83, 6391-6403
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Neuropathogenesis of Congenital Cytomegalovirus Infection: Disease Mechanisms and Prospects for Intervention.
M. C.-J. Cheeran, J. R. Lokensgard, and M. R. Schleiss (2009)
Clin. Microbiol. Rev. 22, 99-126
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Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression.
I. J. Groves and J. H. Sinclair (2007)
J. Gen. Virol. 88, 2935-2940
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Human Cytomegalovirus Gene Expression Is Silenced by Daxx-Mediated Intrinsic Immune Defense in Model Latent Infections Established In Vitro.
R. T. Saffert and R. F. Kalejta (2007)
J. Virol. 81, 9109-9120
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Reversal of Human Cytomegalovirus Major Immediate-Early Enhancer/Promoter Silencing in Quiescently Infected Cells via the Cyclic AMP Signaling Pathway.
M. J. Keller, A. W. Wu, J. I. Andrews, P. W. McGonagill, E. E. Tibesar, and J. L. Meier (2007)
J. Virol. 81, 6669-6681
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Epstein-Barr Virus Lytic Infection Induces Retinoic Acid-responsive Genes through Induction of a Retinol-metabolizing Enzyme, DHRS9.
R. J. Jones, S. Dickerson, P. M. Bhende, H.-J. Delecluse, and S. C. Kenney (2007)
J. Biol. Chem. 282, 8317-8324
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Latency and reactivation of human cytomegalovirus.
J. Sinclair and P. Sissons (2006)
J. Gen. Virol. 87, 1763-1779
   Abstract »    Full Text »    PDF »
Ets-2 repressor factor recruits histone deacetylase to silence human cytomegalovirus immediate-early gene expression in non-permissive cells.
E. Wright, M. Bain, L. Teague, J. Murphy, and J. Sinclair (2005)
J. Gen. Virol. 86, 535-544
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Human Cytomegalovirus Induces Inhibition of Macrophage Differentiation by Binding to Human Aminopeptidase N/CD13.
S. Gredmark, W. B. Britt, X. Xie, L. Lindbom, and C. Soderberg-Naucler (2004)
J. Immunol. 173, 4897-4907
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Human Cytomegalovirus Inhibits Cytokine-Induced Macrophage Differentiation.
S. Gredmark, T. Tilburgs, and C. Soderberg-Naucler (2004)
J. Virol. 78, 10378-10389
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Role of the Human Cytomegalovirus Major Immediate-Early Promoter's 19-Base-Pair-Repeat Cyclic AMP-Response Element in Acutely Infected Cells.
M. J. Keller, D. G. Wheeler, E. Cooper, and J. L. Meier (2003)
J. Virol. 77, 6666-6675
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Ets-2 Repressor Factor (ERF) mediates repression of the human cytomegalovirus major immediate-early promoter in undifferentiated non-permissive cells.
M. Bain, M. Mendelson, and J. Sinclair (2003)
J. Gen. Virol. 84, 41-49
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Infection of Cells with Human Cytomegalovirus during S Phase Results in a Blockade to Immediate-Early Gene Expression That Can Be Overcome by Inhibition of the Proteasome.
E. A. Fortunato, V. Sanchez, J. Y. Yen, and D. H. Spector (2002)
J. Virol. 76, 5369-5379
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Late temporal gene expression from the human cytomegalovirus pp28US (UL99) promoter when integrated into the host cell chromosome.
J. Wu, J. O’Neill, and M. S. Barbosa (2001)
J. Gen. Virol. 82, 1147-1155
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Human telomerase reverse transcriptase-immortalized MRC-5 and HCA2 human fibroblasts are fully permissive for human cytomegalovirus.
B. P. McSharry, C. J. Jones, J. W. Skinner, D. Kipling, and G. W. G. Wilkinson (2001)
J. Gen. Virol. 82, 855-863
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Reactivation of the Human Cytomegalovirus Major Immediate-Early Regulatory Region and Viral Replication in Embryonal NTera2 Cells: Role of Trichostatin A, Retinoic Acid, and Deletion of the 21-Base-Pair Repeats and Modulator.
J. L. Meier (2001)
J. Virol. 75, 1581-1593
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The Human Cytomegalovirus IE86 Protein Can Block Cell Cycle Progression after Inducing Transition into the S Phase of Permissive Cells.
E. A. Murphy, D. N. Streblow, J. A. Nelson, and M. F. Stinski (2000)
J. Virol. 74, 7108-7118
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Human cytomegalovirus mediates cell cycle progression through G1 into early S phase in terminally differentiated cells.
J. Sinclair, J. Baillie, L. Bryant, and R. Caswell (2000)
J. Gen. Virol. 81, 1553-1565
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The Human Cytomegalovirus Major Immediate-Early Distal Enhancer Region Is Required for Efficient Viral Replication and Immediate-Early Gene Expression.
J. L. Meier and J. A. Pruessner (2000)
J. Virol. 74, 1602-1613
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Human Cytomegalovirus Infection of Caco-2 Cells Occurs at the Basolateral Membrane and Is Differentiation State Dependent.
M. A. Jarvis, C. E. Wang, H. L. Meyers, P. P. Smith, C. L. Corless, G. J. Henderson, J. Vieira, W. J. Britt, and J. A. Nelson (1999)
J. Virol. 73, 4552-4560
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Phosphorylation of the Human Cytomegalovirus 86-Kilodalton Immediate-Early Protein IE2.
N. Y. Harel and J. C. Alwine (1998)
J. Virol. 72, 5481-5492
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Ligand Induction of Retinoic Acid Receptors Alters an Acute Infection by Murine Cytomegalovirus.
A. Angulo, R. A. S. Chandraratna, J. F. LeBlanc, and P. Ghazal (1998)
J. Virol. 72, 4589-4600
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