Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
American Society for Biochemistry and Molecular Biology

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 6 April 1984:
Vol. 224. no. 4644, pp. 80 - 83
DOI: 10.1126/science.224.4644.80
Prev | Table of Contents | Next

Articles

An Unusual Phycoerythrin from a Marine Cyanobacterium

LINDA J. ONG 1, ALEXANDER N. GLAZER 1, and JOHN B. WATERBURY 2

1 Department of Microbiology and Immunology, University of California, Berkeley 94720
2 Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543

Phycoerythrin conjugates are reagents for cell sorting and analyses in which the argon-ion laser line at 488 nanometers is used for excitation. Many marine Synechococcus strains contain phycoerythrins with absorption maxima at approximately 490 and 550 nanometers; these maxima indicate the presence of phycourobilin and phycoerythrobilin prosthetic groups in the protein. Phycoerythrins of red algae contain both groups, but those of freshwater and soil cyanobacteria contain only phycoerythrobilin. Phycoerythrin purified from Synechococcus WH8103 has molecular properties typical of red algal phycoerythrins, but its phycourobilin content is higher than that of other phycoerythrins. The protein has absorption maxima at 492 and 543 nanometers and corresponding molar extinction coefficients of 2.78 and 1.14 x 106; it fluoresces maximally at 565 nanometers with a quantum yield of 0.5. Conjugates of Synechococcus WH8103 phycoerythrin could increase the sensitivity of cell analysis techniques to almost twice that possible with other phycoerythrin conjugates.

Submitted on January 6, 1984
Accepted on February 8, 1984


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Two Novel Phycoerythrin-Associated Linker Proteins in the Marine Cyanobacterium Synechococcus sp. Strain WH8102.
C. Six, J.-C. Thomas, L. Thion, Y. Lemoine, F. Zal, and F. Partensky (2005)
J. Bacteriol. 187, 1685-1694
   Abstract »    Full Text »    PDF »
Unique Fluorescence Properties of a Cyanobacterium Gloeobacter violaceus PCC 7421: Reasons for Absence of the Long-Wavelength PSI Chl a Fluorescence at -196{degrees}C.
M. Mimuro, T. Ookubo, D. Takahashi, T. Sakawa, S. Akimoto, I. Yamazaki, and H. Miyashita (2002)
Plant Cell Physiol. 43, 587-594
   Abstract »    Full Text »    PDF »
Diel Rhythms in Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Glutamine Synthetase Gene Expression in a Natural Population of Marine Picoplanktonic Cyanobacteria (Synechococcus spp.).
M. Wyman (1999)
Appl. Envir. Microbiol. 65, 3651-3659
   Abstract »    Full Text »
The Discovery of a Novel R-phycoerythrin from an Antarctic Red Alga.
R. MacColl, L. E. Eisele, E. C. Williams, and S. S. Bowser (1996)
J. Biol. Chem. 271, 17157-17160
   Abstract »    Full Text »    PDF »
Role of phycoerythrin in marine picoplankton Synechococcus spp.
S. Yeh, L. Ong, and A. Glazer (1986)
Science 234, 1422-1424
   PDF »
Novel Role for Phycoerythrin in a Marine Cyanobacterium, Synechococcus Strain DC2.
M. WYMAN, R. P. F. GREGORY, and N. G. CARR (1985)
Science 230, 818-820
   Abstract »    PDF »
A Cyanobacterium Capable of Swimming Motility.
J. B. WATERBURY, J. M. WILLEY, D. G. FRANKS, F. W. VALOIS, and S. W. WATSON (1985)
Science 230, 74-76
   Abstract »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)