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Science 23 March 1984:
Vol. 223. no. 4642, pp. 1299 - 1301
DOI: 10.1126/science.6322300
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Articles

Science, Vol 223, Issue 4642, 1299-1301
Copyright © 1984 by American Association for the Advancement of Science

articles

Total synthesis and cloning of a gene coding for the ribonuclease S protein

KP Nambiar, J Stackhouse, DM Stauffer, WP Kennedy, JK Eldredge, and SA Benner

A gene for ribonuclease S protein, has been chemically synthesized and cloned. The gene is designed to have 25 specific restriction endonuclease sites spaced at short intervals, permitting its structure to be rapidly modified. This flexibility facilitates tests of hypotheses relating the primary structure of the enzyme to its physical and catalytic behavior.


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