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Science 23 March 1984: Vol. 223. no. 4642, pp. 1296 - 1299 DOI: 10.1126/science.6199844
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Articles
Science, Vol 223, Issue 4642, 1296-1299
Copyright © 1984 by American Association for the Advancement of Science
Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor
Y Shing,
J Folkman,
R Sullivan,
C Butterfield,
J Murray,
and
M Klagsbrun
A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.
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