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Science 17 February 1984:
Vol. 223. no. 4637, pp. 661 - 664
DOI: 10.1126/science.6695174
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Articles

Science, Vol 223, Issue 4637, 661-664
Copyright © 1984 by American Association for the Advancement of Science

articles

Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient

E Santos, D Martin-Zanca, EP Reddy, MA Pierotti, G Della Porta, and M Barbacid

A single genetic alteration, a guanine-to-cytosine transversion, is responsible for the acquisition of malignant properties by K-ras genes of two human tumor cell lines established from carcinomas of the bladder (A1698) and lung (A2182). As a consequence, arginine instead of the normal glycine is incorporated into the K-ras-coded p21 proteins at amino acid position 12. This mutation creates a restriction enzyme polymorphism that can be used to screen human cells for transforming K-ras genes. This approach was used to identify the mutational event responsible for the malignant activation of a K-ras oncogene in a squamous cell lung carcinoma of a 66-year-old man; this point mutation was not present in either the normal bronchial or parenchymal tissue or in the blood lymphocytes. Hence, malignant activation of a ras oncogene appears to be specifically associated with the development of a human neoplasm.


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