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Science 9 December 1983:
Vol. 222. no. 4628, pp. 1144 - 1146
DOI: 10.1126/science.6648529
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Articles

Science, Vol 222, Issue 4628, 1144-1146
Copyright © 1983 by American Association for the Advancement of Science

articles

Transformation of Bloom's syndrome fibroblasts by DNA transfection

J Doniger, JA Di Paolo, and NC Popescu

Nonmalignant diploid human fibroblast cells (GM3498B) derived from a skin biopsy of a patient with Bloom's syndrome have been transformed by transfection with DNA from a tumorigenic mouse cell line (Ha-8) carrying a single copy of the Harvey murine sarcoma virus (Ha-MuSV) genome. The transformed cell lines have an extended life-span, form colonies in agarose, and proliferate in nude mice--characteristics of neoplastic transformation. Like the parental cells, they also exhibit a high spontaneous level of sister chromatid exchanges. Finally, the transformed cells contain most, if not all, of the Ha-MuSV genome as well as the human rasH sequence. These experiments show that these diploid nonmalignant human cells can be used as recipients in transfection experiments for studying the genetic control of neoplastic transformation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Transformation of human bronchial epithelial cells transfected by Harvey ras oncogene.
G. Yoakum, J. Lechner, E. Gabrielson, B. Korba, L Malan-Shibley, J. Willey, M. Valerio, A. Shamsuddin, B. Trump, and C. Harris (1985)
Science 227, 1174-1179
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