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Science 9 December 1983:
Vol. 222. no. 4628, pp. 1141 - 1143
DOI: 10.1126/science.6648528
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Articles

Science, Vol 222, Issue 4628, 1141-1143
Copyright © 1983 by American Association for the Advancement of Science

articles

X-ray--induced breakage and rejoining of human interphase chromosomes

MN Cornforth and JS Bedford

A method was developed for the high-resolution measurement of breaks in prematurely condensed chromosomes at the G1 phase of the cell cycle. The dose response for fragments (breaks) produced immediately after x-irradiation of confluent cultures of normal human cells was linear down to 10.9 rad (0.109 Gy) and extrapolated to zero effect at zero dose. The curve had a slope of 0.063 breaks per cell per rad, which is at least an order of magnitude greater than that for breaks scored in the same cells after they have progressed to mitosis following subculture. When incubated at 37 degrees C half of the breaks disappeared in 2 hours. A slower, perhaps nonrejoining component was apparent at later incubation times. The initial rate of break rejoining was similar to the rate of increase in survival after incubation because of the repair of potentially lethal damage and is also in close agreement with recently reported values for the rejoining of double-strand breakage in DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pre-irradiation exposure of peripheral blood lymphocytes to glutaraldehyde induces radiosensitization by increasing the initial yield of radiation-induced chromosomal aberrations.
V. I. Hatzi, G. I. Terzoudi, V. Makropoulos, C. Maravelias, and G. E. Pantelias (2008)
Mutagenesis 23, 101-109
   Abstract »    Full Text »    PDF »
Silencing Expression of the Catalytic Subunit of DNA-dependent Protein Kinase by Small Interfering RNA Sensitizes Human Cells for Radiation-induced Chromosome Damage, Cell Killing, and Mutation.
Y. Peng, Q. Zhang, H. Nagasawa, R. Okayasu, H. L. Liber, and J. S. Bedford (2002)
Cancer Res. 62, 6400-6404
   Abstract »    Full Text »    PDF »
Chromosome Instability in Lymphocytes: A Potential Indicator of Predisposition to Oral Premalignant Lesions.
X. Wu, S. M. Lippman, J. J. Lee, Y. Zhu, Q. V. Wei, M. Thomas, W. K. Hong, and M. R. Spitz (2002)
Cancer Res. 62, 2813-2818
   Abstract »    Full Text »    PDF »
Radiosensitivity in Nijmegen Breakage Syndrome Cells Is Attributable to a Repair Defect and not Cell Cycle Checkpoint Defects.
P.-M. Girard, N. Foray, M. Stumm, A. Waugh, E. Riballo, R. S. Maser, W. P. Phillips, J. Petrini, C. F. Arlett, and P. A. Jeggo (2000)
Cancer Res. 60, 4881-4888
   Abstract »    Full Text »
On the nature of a defect in cells from individuals with ataxia-telangiectasia.
M. Cornforth and J. Bedford (1985)
Science 227, 1589-1591
   Abstract »    PDF »


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