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Science 9 December 1983:
Vol. 222. no. 4628, pp. 1129 - 1132
DOI: 10.1126/science.6648524
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Articles

Science, Vol 222, Issue 4628, 1129-1132
Copyright © 1983 by American Association for the Advancement of Science

articles

Monoclonal antibodies to a synthetic fibrin-like peptide bind to human fibrin but not fibrinogen

KY Hui, E Haber, and GR Matsueda

A synthetic heptapeptide from the amino terminus of the beta chain in human fibrin was used as an antigen to produce monoclonal antibodies that bind to fibrin even in the presence of human fibrinogen at the concentration found in plasma. As expected, the antifibrin activity was inhibited by the peptide antigen but not by a control heptapeptide. In a chicken ex vivo circulatory model for fibrin detection, intravenously administered monoclonal antibodies bound to human fibrin-coated disks placed in an extracorporeal chamber. These findings may lead to better methods for identifying deep vein and coronary artery thrombi.


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