Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 2 December 1983:
Vol. 222. no. 4627, pp. 1025 - 1027
DOI: 10.1126/science.6648515
Prev | Table of Contents | Next

Articles

Science, Vol 222, Issue 4627, 1025-1027
Copyright © 1983 by American Association for the Advancement of Science

articles

Identification of presynaptic neurons by laser photostimulation

IC Farber and A Grinvald

An optical method involving the use of a laser and a novel fluorescent dye as a photostimulation probe has been developed to identify presynaptic neurons in a large ensemble of cells. Illumination of an extracellularly stained neuron by the laser microbeam evokes action potentials. With this technique an interneuron connecting identified leech neurons was quickly located. The method speeds up the elucidation of neuronal networks, especially when small cells are involved.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Optical probing of neuronal circuits with calcium indicators.
Z. A. Peterlin, J. Kozloski, B.-Q. Mao, A. Tsiola, and R. Yuste (2000)
PNAS 97, 3619-3624
   Abstract »    Full Text »    PDF »
Rearrangements of synaptic connections in visual cortex revealed by laser photostimulation.
M. Dalva and L. Katz (1994)
Science 265, 255-258
   Abstract »    PDF »
Phase I and phase II of short-term mechanical restitution in perfused rat left ventricles.
C. Dumitrescu, P. Narayan, Y. Cheng, I. R. Efimov, and R. A. Altschuld (2002)
Am J Physiol Heart Circ Physiol 282, H1311-H1319
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)