Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 11 November 1983:
Vol. 222. no. 4624, pp. 632 - 634
DOI: 10.1126/science.222.4624.632
Prev | Table of Contents | Next

Articles

A Morphogenetically Competent Soybean Suspension Culture

M. L. CHRISTIANSON 1, D. A. WARNICK 1, and P. S. CARLSON 1

1 Department of Molecular Biology, Zoecon Corporation, Palo Alto, California 94304

A morphogenetically competent suspension culture was derived from embryonic axes of Glycine max cv. Mitchell. The cultural history included visual selection for nonfriable, embryo-like structures, recurrent selection in a regime of 2,4-dichlorophenoxyacetic acid exposure and withdrawal, and the replacement of the nitrogen in a Murashige and Skoog salts-based medium with 20 millimolar ammonium citrate. The embryoids produced by this suspension are capable of completing plantlet development. The suspension can be maintained by serial subculture.

Submitted on March 22, 1983
Revised on April 25, 1983

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)