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Science 11 November 1983: Vol. 222. no. 4624, pp. 623 - 625 DOI: 10.1126/science.6635659
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Articles
Science, Vol 222, Issue 4624, 623-625
Copyright © 1983 by American Association for the Advancement of Science
Human endothelial cells: use of heparin in cloning and long-term serial cultivation
SC Thornton,
SN Mueller,
and
EM Levine
Endothelial cells from human blood vessels were cultured in vitro, with doubling times of 17 to 21 hours for 42 to 79 population doublings. Cloned human endothelial cell strains were established for the first time and had similar proliferative capacities. This vigorous cell growth was achieved by addition of heparin to culture medium containing reduced concentrations of endothelial cell growth factor. The routine cloning and long-term culture of human endothelial cells will facilitate studying the human endothelium in vitro.
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