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Science 28 October 1983:
Vol. 222. no. 4622, pp. 385 - 389
DOI: 10.1126/science.6194563
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Articles

Science, Vol 222, Issue 4622, 385-389
Copyright © 1983 by American Association for the Advancement of Science

articles

High-frequency transfection and cytopathology of the hepatitis B virus core antigen gene in human cells

GH Yoakum, BE Korba, JF Lechner, T Tokiwa, AF Gazdar, T Seeley, M Siegel, L Leeman, H Autrup, and CC Harris

A protoplast fusion method was developed to stably transfect human cells with pSV2-derived plasmids at frequencies greater than 10(-3). This procedure made it possible to test the biological effect of a hepatitis B virus (HBV) gene independent of the viral structures required for infection. A pSV2gpt+ plasmid constructed to carry a subgenomic fragment of HBV that contained the core antigen gene (HBc gene) was transfected into human cells. A human epithelial cell line was stably transfected with the HBc+ gene by selecting recipient cells for expression of guanine phosphoribosyl transferase expression. With this gpt+/HBc+ cell line it was shown that growth in serum-free medium or treatment with 5'-azacytidine stimulates the production of the HBV core antigen. A hepatocellular carcinoma carrying the entire HBV genome was stimulated to produce the HBc gene product in response to the same factors that stimulated HBcAg production in the gpt+/HBc+ cell line constructed by transfection. The temporal relation between the cytopathologic response and HBc gene expression was similar for both cell types, indicating a primary role for HBc gene expression in the cytopathology of HBV-infected human liver.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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