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Science 7 October 1983:
Vol. 222. no. 4619, pp. 62 - 65
DOI: 10.1126/science.6353573
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Articles

Science, Vol 222, Issue 4619, 62-65
Copyright © 1983 by American Association for the Advancement of Science

articles

In vivo determination of the pyridine nucleotide reduction charge by carbon-13 nuclear magnetic resonance spectroscopy

CJ Unkefer, RM Blazer, and RE London

An intracellular coenzyme has been observed by carbon-13 nuclear magnetic resonance spectroscopy. The pyridine nucleotides in Escherichia coli were specifically labeled with carbon-13 from the biosynthetic precursor, nicotinic acid. The intracellular redox status and metabolic transformations of the pyridine nucleotides were examined under a variety of conditions. A highly reduced nicotinamide adenine dinucleotide pool was observed under anaerobic conditions only in cells that were cultured aerobically on glycerol.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Is the Glycolytic Flux in Lactococcus lactis Primarily Controlled by the Redox Charge? KINETICS OF NAD+ AND NADH POOLS DETERMINED IN VIVO BY 13C NMR.
A. R. Neves, R. Ventura, N. Mansour, C. Shearman, M. J. Gasson, C. Maycock, A. Ramos, and H. Santos (2002)
J. Biol. Chem. 277, 28088-28098
   Abstract »    Full Text »    PDF »
Studying enzyme mechanism by 13C nuclear magnetic resonance.
N. Mackenzie, J. Malthouse, and A. Scott (1984)
Science 225, 883-889
   Abstract »    PDF »


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