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Science 30 September 1983:
Vol. 221. no. 4618, pp. 1413 - 1415
DOI: 10.1126/science.6310757
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Articles

Science, Vol 221, Issue 4618, 1413-1415
Copyright © 1983 by American Association for the Advancement of Science

articles

Is cytosolic ionized calcium regulating neutrophil activation?

T Pozzan, DP Lew, CB Wollheim, and RY Tsien

The concentration of cytosolic ionized calcium, [Ca2+]i, was measured in intact neutrophils by use of a fluorescent indicator trapped in the icytoplasm. A given rise of [Ca2+]i elicited by the chemotactic peptide formylmethionylleucylphenylalanine (FMLP) was associated with a much greater degree of superoxide generation and myeloperoxidase secretion than was the same or larger [Ca2+]i produced by a specific calcium ionophore, ionomycin, which bypasses cell surface receptors. Thus, FMLP appears to generate some important excitatory signal in addition to a rise in [Ca2+]i and exocytosis and superoxide generation in neutrophils may not be simply dependent on [Ca2+]i as is widely supposed.


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