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Science 30 September 1983:
Vol. 221. no. 4618, pp. 1348 - 1350
DOI: 10.1126/science.6310754
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Articles

Science, Vol 221, Issue 4618, 1348-1350
Copyright © 1983 by American Association for the Advancement of Science

articles

Expression of a platelet-derived growth factor-like protein in simian sarcoma virus transformed cells

TF Deuel, JS Huang, SS Huang, P Stroobant, and MD Waterfield

The near identity of the partial amino acid sequence of human platelet-derived growth factor (PDGF) and that predicted for p28sis, the putative transforming protein of the simian sarcoma virus (SSV), suggests expression of a growth factor activity may be central for transformation by SSV. It is now reported that SSV-transformed cells but not control cells contain a growth factor activity that is identical to PDGF in immunoassay, in mitogenic dose response, and in specific mitogenic activity. The protein immunoprecipitated by antiserum to human PDGF has an apparent molecular weight of 20,000, identical to that of p20sis, the putative intracellular degradation product of p28sis. The results support the concept that expression of a PDGF-like molecule, which appears to be the product of the viral-sis gene, is responsible for the abnormal regulation of growth is SSV-transformed cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification of a Thrombin Response Element in the Human Platelet-derived Growth Factor B-chain (c-sis) Promoter.
E. M. Scarpati and P. E. DiCorleto (1996)
J. Biol. Chem. 271, 3025-3032
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Mesenchymal Cell Growth Factors.
D. T. Graves and D. L. Cochran (1990)
Critical Reviews in Oral Biology & Medicine 1, 17-36
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Transformation by v-sis occurs by an internal autoactivation mechanism.
B. Bejcek, D. Li, and T. Deuel (1989)
Science 245, 1496-1499
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Diminished response of Werner's syndrome fibroblasts to growth factors PDGF and FGF.
E. Bauer, N Silverman, D. Busiek, A Kronberger, and T. Deuel (1986)
Science 234, 1240-1243
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Microinjected c-myc as a competence factor.
L Kaczmarek, J. Hyland, R Watt, M Rosenberg, and R Baserga (1985)
Science 228, 1313-1315
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Requirement for a signal sequence in biological expression of the v-sis oncogene.
M Hannink and D. Donoghue (1984)
Science 226, 1197-1199
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Expression of the c-fos gene and of an fos-related gene is stimulated by platelet-derived growth factor.
B. Cochran, J Zullo, I. Verma, and C. Stiles (1984)
Science 226, 1080-1082
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Detection of high molecular weight forms of platelet-derived growth factor by sequence-specific antisera.
H. Niman, R. Houghten, and D. Bowen-Pope (1984)
Science 226, 701-703
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Transforming potential of human c-sis nucleotide sequences encoding platelet-derived growth factor.
S. Josephs, L Ratner, M. Clarke, E. Westin, M. Reitz, and F Wong-Staal (1984)
Science 225, 636-639
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Simian sarcoma virus--transformed cells secrete a mitogen identical to platelet-derived growth factor.
A. Owen, P Pantazis, and H. Antoniades (1984)
Science 225, 54-56
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Human-proto-oncogene nucleotide sequences corresponding to the transforming region of simian sarcoma virus.
S. Josephs, C Guo, L Ratner, and F Wong-Staal (1984)
Science 223, 487-491
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Platelet-derived Growth Factor (PDGF) Receptor-alpha Activates c-Jun NH2-terminal Kinase-1 and Antagonizes PDGF Receptor-beta -induced Phenotypic Transformation.
J. Yu, T. F. Deuel, and H.-R. C. Kim (2000)
J. Biol. Chem. 275, 19076-19082
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