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Science 23 September 1983:
Vol. 221. no. 4617, pp. 1288 - 1290
DOI: 10.1126/science.6612343
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Articles

Science, Vol 221, Issue 4617, 1288-1290
Copyright © 1983 by American Association for the Advancement of Science

articles

Turnover, membrane insertion, and degradation of sodium channels in rabbit urinary bladder

DD Loo, SA Lewis, MS Ifshin, and JM Diamond

Noise analysis of rabbit bladder revealed two components: Lorentzian noise, arising from interaction of amiloride with the Na+ channel, and flicker noise (l/f, where f is frequency), as in other biological membranes. Hydrostatic pressure, which causes exchange between intracellular vesicular membrane and apical membrane, increases the number but not the single-channel current of the amiloride-sensitive channels. Flicker noise arises from degraded channels that have lost amiloride sensitivity and Na+ to K+ selectivity. The degraded channels were selectively removed by washing the mucosal surface. These results imply channel turnover by intracellular synthesis, transfer from vesicular to apical membrane, degradation, and elimination.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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