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Science 2 September 1983:
Vol. 221. no. 4614, pp. 949 - 951
DOI: 10.1126/science.221.4614.949

Articles

Single Gene Mutations in Tomato Plants Regenerated from Tissue Culture

DAVID A. EVANS 1 and WILLIAM R. SHARP 1

1 DNA Plant Technology Corporation, Cinnaminson, New Jersey 08077

Plants were regenerated from cultured leaf explants of an inbred variety of Lycopersicon esculentum. Seeds were collected from the regenerated plants and sown in the greenhouse. The resultant plants were then evaluated in the field. Several monogenic mutations segregated in the progeny of regenerated plants. The recovery of single gene mutations is evidence that plant tissue culture can be mutagenic. Complementation tests revealed that one mutation was located on the long arm of chromosome 10.

Submitted on November 22, 1982
Revised on January 11, 1983


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Dwarf Mutant of Arabidopsis Generated by T-DNA Insertion Mutagenesis.
K. A. Feldmann, M. D. Marks, M. L. Christianson, and R. S. Quatrano (1989)
Science 243, 1351-1354
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Biotechnology in Food Production and Processing.
D. Knorr and A. J. Sinskey (1985)
Science 229, 1224-1229
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