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Science 26 August 1983:
Vol. 221. no. 4613, pp. 873 - 875
DOI: 10.1126/science.6308765
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Articles

Science, Vol 221, Issue 4613, 873-875
Copyright © 1983 by American Association for the Advancement of Science

articles

Measurement of suppressor transfer RNA activity

JF Young, M Capecchi, FA Laski, UL RajBhandary, PA Sharp, and P Palese

Transfer RNA (tRNA) suppression of nonsense mutations in prokaryotic systems has been widely used to study the structure and function of different prokaryotic genes. Through genetic engineering techniques, it is now possible to introduce suppressor (Su+) tRNA molecules into mammalian cells. A quantitative assay of the suppressor tRNA activity in these mammalian cells is described; it is based on the amount of tRNA-mediated readthrough of a terminating codon in the influenza virus NS1 gene after the cells are infected with virus. Suppressor activity in L cells continuously expressing Su+ (tRNAtyr) was 3.5 percent and that in CV-1 cells infected with an SV40- Su+ (tRNAtyr) recombinant was 22.5 percent.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells.
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Infrequent Translation of a Nonsense Codon Is Sufficient to Decrease mRNA Level.
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An Engineered Tetrahymena tRNAGln for in Vivo Incorporation of Unnatural Amino Acids into Proteins by Nonsense Suppression.
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