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Science 26 August 1983:
Vol. 221. no. 4613, pp. 855 - 858
DOI: 10.1126/science.6348945
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Articles

Science, Vol 221, Issue 4613, 855-858
Copyright © 1983 by American Association for the Advancement of Science

articles

Nucleotide sequence and expression of the diphtheria tox228 gene in Escherichia coli

M Kaczorek, F Delpeyroux, N Chenciner, RE Streeck, Murphy JR, P Boquet, and P Tiollais

The complete nucleotide sequence of the diphtheria tox228 gene encoding the nontoxic serologically related protein CRM228 has been determined. A comparison of the predicted amino acid sequence with the available amino acid sequences from the wild-type toxin made it possible to deduce essentially the entire nucleotide sequence of the wild-type tox gene. The signal peptide of pro-diphtheria toxin and the putative tox promoter have been identified, a highly symmetrical nucleotide sequence downstream of the toxin gene has been detected; this region may be the corynebacteriophage beta attachment site (attP). The cloned toxin gene was expressed at a low level in Escherichia coli.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Increasing Anticarcinoma Activity of an Anti-erbB2 Recombinant Immunotoxin by the Addition of an Anti-EpCAM sFv.
B. J. Stish, H. Chen, Y. Shu, A. Panoskaltsis-Mortari, and D. A. Vallera (2007)
Clin. Cancer Res. 13, 3058-3067
   Abstract »    Full Text »    PDF »
Pertussis toxin gene: nucleotide sequence and genetic organization.
C Locht and J. Keith (1986)
Science 232, 1258-1264
   Abstract »    PDF »


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