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Science 26 August 1983:
Vol. 221. no. 4613, pp. 850 - 851
DOI: 10.1126/science.221.4613.850
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Articles

Direct Embryogenesis from Mesophyll Cells of Orchardgrass

B. V. CONGER 1, G. E. HANNING 1, D. J. GRAY 1, and J. K. MCDANIEL 1

1 Department of Plant and Soil Science, University of Tennessee, Post Office Box 1071, Knoxville 37901

Segments taken from the basal 15 to 20 millimeters of the two innermost leaves of an orchardgrass (Dactylis glomerata L.) genotype produced somatic (nonzygotic) embryos directly from mesophyll cells without an intervening callus when cultured on an agar medium with 30 micromolar 3,6-dichloro-o-anisic acid (dicamba). This demonstration of high-frequency embryogenesis from mesophyll cells in Gramineae is strong evidence for totipotency of the cells.

Submitted on April 19, 1983
Revised on May 23, 1983

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Science. ISSN 0036-8075 (print), 1095-9203 (online)