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Science 19 August 1983:
Vol. 221. no. 4612, pp. 749 - 750
DOI: 10.1126/science.6879173
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Articles

Science, Vol 221, Issue 4612, 749-750
Copyright © 1983 by American Association for the Advancement of Science

articles

Free-metal ion depletion by "Good's" buffers

R Nakon and CR Krishnamoorthy

Metal-ion affinity (formation) constants were determined for two "Good's" buffers, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) and N,N-bis(2-hydroxyethyl)glycine (bicine). The metal chelates formed undergo loss of an internal ligand (alcohol) proton (bicine) and undergo hydrolysis (bicine and TES) and dimerization reactions (TES). Bicine and TES buffer not only hydrogen ions but also metal ions. The metal complexes of "Good's" buffers also buffer hydrogen ions by secondary reactions. The consequences of these reactions are considered in relation to biomedical research.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)