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Science 22 July 1983:
Vol. 221. no. 4608, pp. 368 - 370
DOI: 10.1126/science.6408733
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Articles

Science, Vol 221, Issue 4608, 368-370
Copyright © 1983 by American Association for the Advancement of Science

articles

Depletion of intracellular polyamines may alter DNA conformation in 9L rat brain tumor cells

DT Hung, LJ Marton, DF Deen, and RH Shafer

Depletion of polyamines in 9L rat brain tumor cells by treatment with alpha-difluoromethylornithine dramatically altered DNA conformation as measured by viscoelastometry. The reduction of intracellular putrescine and spermidine concentrations to less than 5 percent of their concentrations in control cells decreased the sensitivity of 9L cell DNA to x-irradiation and increased the maximum viscoelastic retardation time of the DNA. Both of these phenomena were reversed by addition of exogenous putrescine.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Phase III Randomized Study of Postradiotherapy Chemotherapy with Combination {alpha}-Difluoromethylornithine-PCV versus PCV for Anaplastic Gliomas.
V. A. Levin, K. R. Hess, A. Choucair, P. J. Flynn, K. A. Jaeckle, A. P. Kyritsis, W. K. A. Yung, M. D. Prados, J. M. Bruner, S. Ictech, et al. (2003)
Clin. Cancer Res. 9, 981-990
   Abstract »    Full Text »    PDF »
alpha-Difluoromethylornithine-induced polyamine depletion of 9L tumor cells modifies drug-induced DNA cross-link formation.
P. Tofilon, D. Deen, and L. Marton (1983)
Science 222, 1132-1135
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)