Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 15 July 1983:
Vol. 221. no. 4607, pp. 281 - 283
DOI: 10.1126/science.6190230
Prev | Table of Contents | Next

Articles

Science, Vol 221, Issue 4607, 281-283
Copyright © 1983 by American Association for the Advancement of Science

articles

Basement membrane collagen: degradation by migrating endothelial cells

T Kalebic, S Garbisa, B Glaser, and LA Liotta

One of the first steps in neovascularization is dissolution of the basement membrane at the point of endothelial outgrowth. An assay was developed to determine whether basement membrane collagens (types IV and V) are degraded by endothelial cells migrating toward a chemotactic stimulus. Fetal bovine endothelial cells were placed on one side of a filter containing the collagen substrate, and a chemoattractant derived from retinal extracts was placed on the opposite side. Degradation of both type IV and type V collagens was observed when the retinal factor was placed on the side of the filter opposite the endothelial cells. Metalloproteinases that cleaved type IV and type V collagens could be extracted from the endothelial cells with detergents. Such endothelial cell-associated (possibly membrane-bound) proteinases may locally disrupt the basement membrane and facilitate the outgrowth of capillary sprouts toward the angiogenic stimulus.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pathogenic angiogenesis in IBD and experimental colitis: new ideas and therapeutic avenues.
J. H. Chidlow Jr., D. Shukla, M. B. Grisham, and C. G. Kevil (2007)
Am J Physiol Gastrointest Liver Physiol 293, G5-G18
   Abstract »    Full Text »    PDF »
Terbinafine inhibits endothelial cell migration through suppression of the Rho-mediated pathway.
P.-Y. Ho, W.-B. Zhong, Y.-S. Ho, and W.-S. Lee (2006)
Mol. Cancer Ther. 5, 3130-3138
   Abstract »    Full Text »    PDF »
Angiostatic Effect of Penetrating Ocular Injury: Role of Pigment Epithelium-Derived Factor.
J. S. Penn, G. W. McCollum, J. M. Barnett, X. Q. Werdich, K. A. Koepke, and V. S. Rajaratnam (2006)
Invest. Ophthalmol. Vis. Sci. 47, 405-414
   Abstract »    Full Text »    PDF »
Cytoarchitecture of Choroidal Capillary Endothelial Cells.
R. H. Guymer, A. C. Bird, and G. S. Hageman (2004)
Invest. Ophthalmol. Vis. Sci. 45, 1660-1666
   Abstract »    Full Text »    PDF »
Abnormalities of Basement Membrane on Blood Vessels and Endothelial Sprouts in Tumors.
P. Baluk, S. Morikawa, A. Haskell, M. Mancuso, and D. M. McDonald (2003)
Am. J. Pathol. 163, 1801-1815
   Abstract »    Full Text »    PDF »
Role of the Matrix Metalloproteinase and Plasminogen Activator-Plasmin Systems in Angiogenesis.
M. S. Pepper (2001)
Arterioscler. Thromb. Vasc. Biol. 21, 1104-1117
   Abstract »    Full Text »    PDF »
Influence of laser photocoagulation on choroidal capillary cytoarchitecture.
R. H Guymer, G. S Hageman, and A. C Bird (2001)
Br. J. Ophthalmol. 85, 40-46
   Abstract »    Full Text »
Increased gelatinolytic activity in bronchoalveolar lavage fluid in stable lung transplant recipients.
C. Trello, D. Williams, C. Keller, C Crim, R. Webster, and J. Ohar (1997)
Am. J. Respir. Crit. Care Med. 156, 1978-1986
   Abstract »    Full Text »
The Regulation of Neovascularization by Matrix Metalloproteinases and Their Inhibitors.
M. A. Moses (1997)
Stem Cells 15, 180-189
   Abstract »    Full Text »
Effects of Dexamethasone and Cell Proliferation on the Expression of Matrix Metalloproteinases in Human Mucosal Normal and Malignant Cells.
M. Kylmaniemi, A. Oikarinen, K. Oikarinen, and T. Salo (1996)
Journal of Dental Research 75, 919-926
   Abstract »    PDF »
Regulation of Vascular Smooth Muscle Cell Migration and Proliferation In Vitro and in Injured Rat Arteries by a Synthetic Matrix Metalloproteinase Inhibitor.
N. Zempo, N. Koyama, R. D. Kenagy, H. J. Lea, and A. W. Clowes (1996)
Arterioscler. Thromb. Vasc. Biol. 16, 28-33
   Abstract »    Full Text »
Thrombin Induces the Activation of Progelatinase A in Vascular Endothelial Cells.
S. Zucker, C. Conner, B. I. DiMassmo, H. Ende, M. Drews, M. Seiki, and W. F. Bahou (1995)
J. Biol. Chem. 270, 23730-23738
   Abstract »    Full Text »    PDF »
Expression of Basement Membrane Type IV Collagen and Type IV Collagenases (MMP-2 and MMP-9) in Human Fetal Teeth.
K. Heikinheimo and T. Salo (1995)
Journal of Dental Research 74, 1226-1234
   Abstract »    PDF »
Matrix Metalloproteinases (MMP-2 and MMP-9) of the Oral Cavity: Cellular Origin and Relationship to Periodontal Status.
M. Makela, T. Salo, V.-J. Uitto, and H. Larjava (1994)
Journal of Dental Research 73, 1397-1406
   Abstract »    PDF »
Matrix Metalloproteinases: A Review.
H. Birkedal-Hansen, W.G.I. Moore, M.K. Bodden, L.J. Windsor, B. Birkedal-Hansen, A. DeCarlo, and J.A. Engler (1993)
Critical Reviews in Oral Biology & Medicine 4, 197-250
   Abstract »    Full Text »    PDF »
Transmission Electron Microscopic Study of a Subretinal Choroidal Neovascular Membrane due to Age-Related Macular Degeneration.
K. M. Gehrs, W. J. Heriot, and E. de Juan Jr (1992)
Arch Ophthalmol 110, 833-837
   Abstract »    PDF »
Identification of an inhibitor of neovascularization from cartilage.
M. Moses, J Sudhalter, and R Langer (1990)
Science 248, 1408-1410
   Abstract »    PDF »
Ultrastructure of Bruch's Membrane After Krypton Laser Photocoagulation: II. Repair of Bruch's Membrane and the Role of Macrophages.
A. Pollack, G. E. Korte, W. J. Heriot, and P. Henkind (1986)
Arch Ophthalmol 104, 1377-1382
   Abstract »    PDF »
Retinal Pigment Epithelial Cells Release an Inhibitor of Neovascularization.
B. M. Glaser, P. A. Campochiaro, J. L. Davis Jr, and M. Sato (1985)
Arch Ophthalmol 103, 1870-1875
   Abstract »    PDF »
Endothelial Cells Release a Chemoattractant for Retinal Pigment Epithelial Cells In Vitro.
P. A. Campochiaro and B. M. Glaser (1985)
Arch Ophthalmol 103, 1876-1880
   Abstract »    PDF »
Heparin binds endothelial cell growth factor, the principal endothelial cell mitogen in bovine brain.
T Maciag, T Mehlman, R Friesel, and A. Schreiber (1984)
Science 225, 932-935
   Abstract »    PDF »
Membrane Type 1-Matrix Metalloproteinase Is Activated during Migration of Human Endothelial Cells and Modulates Endothelial Motility and Matrix Remodeling.
B. G. Galvez, S. Matias-Roman, J. P. Albar, F. Sanchez-Madrid, and A. G. Arroyo (2001)
J. Biol. Chem. 276, 37491-37500
   Abstract »    Full Text »    PDF »
Suppression of angiogenesis by lentiviral delivery of PEX, a noncatalytic fragment of matrix metalloproteinase 2.
A. Pfeifer, T. Kessler, S. Silletti, D. A. Cheresh, and I. M. Verma (2000)
PNAS 97, 12227-12232
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)