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Science 1 July 1983:
Vol. 221. no. 4605, pp. 67 - 69
DOI: 10.1126/science.6857265
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Articles

Science, Vol 221, Issue 4605, 67-69
Copyright © 1983 by American Association for the Advancement of Science

articles

Transmission of integrated sea urchin histone genes by nuclear transplantation in Xenopus laevis

LD Etkin and M Roberts

Sea urchin histone genes contained in a recombinant plasmid pSp102 were microinjected into the cytoplasm of fertilized eggs of Xenopus laevis. By the late blastula stage, plasmid DNA sequences were detected comigrating with the high molecular weight cellular DNA (greater than 48 kilobases). Analysis of the DNA from injected embryos digested with various restriction endonuclease demonstrated that the injected DNA was integrated into the frog genome. Clones of embryos containing the pSp102 DNA sequences were produced by means of nuclear transplantation. Individuals of the same clone contain the pSp102 sequences integrated into similar chromosomal locations. These sites vary between different clones.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Activation of dormant genes in specialized cells.
M. DiBerardino, N. Hoffner, and L. Etkin (1984)
Science 224, 946-952
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