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Science 24 June 1983:
Vol. 220. no. 4604, pp. 1351 - 1355
DOI: 10.1126/science.6304877
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Articles

Science, Vol 220, Issue 4604, 1351-1355
Copyright © 1983 by American Association for the Advancement of Science

articles

RNA splice site selection: evidence for a 5' leads to 3' scanning model

KM Lang and RA Spritz

Human G gamma-globin genes containing tandem duplications of the donor (5') or acceptor (3') RNA splice sites of the second intervening sequence were constructed in order to ascertain the directionality of RNA splice site selection. These genes were introduced into cultured monkey cells, and their transcripts were analyzed. Transcripts of these duplication variants were spliced only at the proximal copy of the duplicated splice sites. These data are consistent with a 5' leads to 3' model of splice site selection.


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U1 snRNP can influence 3'-splice site selection as well as 5'-splice site selection..
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Correct in vivo splicing of the mouse immunoglobulin kappa light-chain pre-mRNA is dependent on 5' splice-site position even in the absence of transcription..
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Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing..
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Mutational Analysis of 3' Splice Site Selection during trans-Splicing.
H. S. Hummel, R. D. Gillespie, and J. Swindle (2000)
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