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Science 10 June 1983:
Vol. 220. no. 4602, pp. 1175 - 1177
DOI: 10.1126/science.6304875
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Articles

Science, Vol 220, Issue 4602, 1175-1177
Copyright © 1983 by American Association for the Advancement of Science

articles

Mutation affecting the 12th amino acid of the c-Ha-ras oncogene product occurs infrequently in human cancer

AP Feinberg, B Vogelstein, MJ Droller, SB Baylin, and BD Nelkin

A point mutation alters the 12th amino acid of the c-Ha-ras oncogene product p21 in a human bladder cancer cell line. This is, at present, the only mutation known to result in a human transforming gene. This mutation may therefore represent a possible target for mutagenesis leading to carcinogenesis in humans. By means of restriction enzyme analysis, 29 human cancers, including 20 primary tumor tissues, derived from organs commonly exposed to environmental carcinogens, were tested for the presence of this mutation. None of ten primary bladder carcinomas exhibited the mutation; nor did nine colon carcinomas or ten carcinomas of the lung. Thus the point mutation affecting the 12th amino acid of the c-Ha-ras gene product, while a valuable model for carcinogenesis, does not appear to play a role in the development of most human epithelial cancers of the bladder, colon, or lung.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Cancer Res. 66, 3401-3408
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Dominant transformation by mutated human ras genes in vitro requires more than 100 times higher expression than is observed in cancers.
V. Y. Hua, W. K. Wang, and P. H. Duesberg (1997)
PNAS 94, 9614-9619
   Abstract »    Full Text »    PDF »
PCR-based approaches for detection of mutated ras genes..
A D van Mansfeld and J L Bos (1992)
Genome Res. 1, 211-216
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Identification of p53 gene mutations in bladder cancers and urine samples.
D Sidransky, A Von Eschenbach, Y. Tsai, P Jones, I Summerhayes, F Marshall, M Paul, P Green, Hamilton SR, P Frost, et al. (1991)
Science 252, 706-709
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Activated proto-onc genes: sufficient or necessary for cancer?.
P. Duesberg (1985)
Science 228, 669-677
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Oncogenes: Implications for the Diagnosis and Treatment of Cancer.
M. J. CLINE, D. J. SLAMON, and J. S. LIPSICK (1984)
Ann Intern Med 101, 223-233
   Abstract »    PDF »
Expression of cellular oncogenes in human malignancies.
D. Slamon, J. deKernion, I. Verma, and M. Cline (1984)
Science 224, 256-262
   Abstract »    PDF »
Malignant activation of a K-ras oncogene in lung carcinoma but not in normal tissue of the same patient.
E Santos, D Martin-Zanca, E. Reddy, M. Pierotti, G Della Porta, and M Barbacid (1984)
Science 223, 661-664
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