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Science 3 June 1983:
Vol. 220. no. 4601, pp. 1049 - 1051
DOI: 10.1126/science.220.4601.1049
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Articles

Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant Tissues

DAVID W. GALBRAITH 1, KRISTI R. HARKINS 1, JOYCE M. MADDOX 1, NICOLA M. AYRES 1, DHARAM P. SHARMA 1, and EBRAHIM FIROOZABADY 1

1 School of Life Sciences, University of Nebraska, Lincoln 68588

Mechanical chopping of plant tissues in the presence of mithramycin released intact nuclei representative of the cells within the tissues. The amount of nuclear DNA in the homogenates of monocotyledonous and dicotyledonous plants was accurately and rapidly determined by flow microfluorometry, and the distribution of nuclei involved in the cell cycle was charted for tissues selected from different physical locations or developmental stages.

Submitted on November 15, 1982
Revised on January 14, 1983


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