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Science 13 May 1983:
Vol. 220. no. 4598, pp. 719 - 721
DOI: 10.1126/science.6601299
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Articles

Science, Vol 220, Issue 4598, 719-721
Copyright © 1983 by American Association for the Advancement of Science

articles

Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components

MJ Lohka and Y Masui

A cell-free preparation of the cytoplasm from activated eggs of Rana pipiens induces, in demembranated sperm nuclei of Xenopus laevis, formation of a nuclear envelope, chromatin decondensation, initiation of DNA synthesis, and chromosome condensation. Both soluble and particulate cytoplasmic constituents are required to initiate these processes in vitro. The observed changes resemble processes occurring during fertilization and the mitotic cycle in early amphibian embryos. Therefore, this cell-free system may be useful in biochemical analysis of the interactions of nucleus and cytoplasm that control nuclear behavior.


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Association of the nucleolar transcription factor UBF with the transcriptionally inactive rRNA genes of pronuclei and early Xenopus embryos.
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J F Diffley (1996)
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ATP-facilitated Chromatin Assembly with a Nucleoplasmin-like Protein from Drosophila melanogaster.
T. Ito, J. K. Tyler, M. Bulger, R. Kobayashi, and J. T. Kadonaga (1996)
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Characterization and quantitation of three B-type lamins in Xenopus oocytes and eggs: increase of lamin LI protein synthesis during meiotic maturation.
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Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts.
U. Strausfeld, M Howell, P Descombes, S Chevalier, R. Rempel, J Adamczewski, J. Maller, T Hunt, and J. Blow (1996)
J. Cell Sci. 109, 1555-1563
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Assembly/Disassembly of the Nuclear Envelope Membrane.
R. Pfaller and J. W. Newport (1995)
J. Biol. Chem. 270, 19066-19072
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Differential Mitotic Phosphorylation of Proteins of the Nuclear Pore Complex.
C. Macaulay, E. Meier, and D. J. Forbes (1995)
J. Biol. Chem. 270, 254-262
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Regulated expression of the beta-globin gene locus in synthetic nuclei..
M C Barton and B M Emerson (1994)
Genes & Dev. 8, 2453-2465
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Redistribution of cytoplasmic components during germinal vesicle breakdown in starfish oocytes.
M. Terasaki (1994)
J. Cell Sci. 107, 1797-1805
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Weaving a pattern from disparate threads: lamin function in nuclear assembly and DNA replication.
C. Hutchison, J. Bridger, L. Cox, and I. Kill (1994)
J. Cell Sci. 107, 3259-3269
   Abstract »    PDF »
Meiosis-specific cell cycle regulation in maturing Xenopus oocytes.
K Ohsumi, W Sawada, and T Kishimoto (1994)
J. Cell Sci. 107, 3005-3013
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Differential replication capacities of G1 and S-phase extracts from sea urchin eggs.
H Zhang and J. Ruderman (1993)
J. Cell Sci. 104, 565-572
   Abstract »    PDF »
The nuclear membrane.
C Dingwall and R Laskey (1992)
Science 258, 942-947
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Control of DNA Replication in Reconstituted Nuclei.
R.A. Laskey, L.S. Cox, G.H. Leno, A. Philpott, A.D. Mills, and A.M. Sleeman (1991)
Cold Spring Harb Symp Quant Biol 56, 347-352
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S phase of the cell cycle.
R. Laskey, M. Fairman, and J. Blow (1989)
Science 246, 609-614
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Dominoes and clocks: the union of two views of the cell cycle.
A. Murray and M. Kirschner (1989)
Science 246, 614-621
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Kinetics and mechanism of DNA uptake into the cell nucleus.
H. Salman, D. Zbaida, Y. Rabin, D. Chatenay, and M. Elbaum (2001)
PNAS 98, 7247-7252
   Abstract »    Full Text »    PDF »


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