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Science 6 May 1983:
Vol. 220. no. 4597, pp. 613 - 615
DOI: 10.1126/science.6836304
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Articles

Science, Vol 220, Issue 4597, 613-615
Copyright © 1983 by American Association for the Advancement of Science

articles

Radioactive labeling of antibody: a simple and efficient method

DJ Hnatowich, WW Layne, RL Childs, D Lanteigne, MA Davis, TW Griffin, and PW Doherty

A simple and efficient method of covalently coupling the strong chelator diethylenetriaminepentaacetic acid to proteins was developed for radiolabeling immunoglobulin G antibodies. After being coupled and labeled with indium-111, a monoclonal antibody to carcinoembryonic antigen retained its ability to bind to its antigen in vitro and in vivo. In nude mice with a human colorectal xenograft, 41 percent of the injected radioactivity became localized in each gram of xenograft at 24 hours compared with 9 percent for control antibody and 19 percent for radioiodinated antibody to carcinoembryonic antigen.


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