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Science 6 May 1983:
Vol. 220. no. 4597, pp. 606 - 609
DOI: 10.1126/science.6301012
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Articles

Science, Vol 220, Issue 4597, 606-609
Copyright © 1983 by American Association for the Advancement of Science

articles

High-efficiency ligation and recombination of DNA fragments by vertebrate cells

CK Miller and HM Temin

DNA-mediated gene transfer (transfection) is used to introduce specific genes into vertebrate cells. Events soon after transfection were quantitatively analyzed by determining the infectivity of the DNA from an avian retrovirus and of mixtures of subgenomic fragments of this DNA. The limiting step of transfection with two DNA molecules is the uptake by a single cell of both DNA's in a biologically active state. Transfected cells mediate ligation and recombination of physically unlinked DNA's at nearly 100 percent efficiency.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Reassessment of the Roles of Integrase and the Central DNA Flap in Human Immunodeficiency Virus Type 1 Nuclear Import.
J. D. Dvorin, P. Bell, G. G. Maul, M. Yamashita, M. Emerman, and M. H. Malim (2002)
J. Virol. 76, 12087-12096
   Abstract »    Full Text »    PDF »
Homologous Recombination between Defective neo Genes in Mouse 3T6 Cells.
A.J.H. Smith and P. Berg (1984)
Cold Spring Harb Symp Quant Biol 49, 171-181
   Abstract »    PDF »
Homologous Recombination in Monkey Cells and Human Cell-free Extracts.
R.S. Kucherlapati, D. Ayares, A. Hanneken, K. Noonan, S. Rauth, J.M. Spencer, L. Wallace, and P.D. Moore (1984)
Cold Spring Harb Symp Quant Biol 49, 191-197
   Abstract »    PDF »


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