Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 8 April 1983:
Vol. 220. no. 4593, pp. 216 - 218
DOI: 10.1126/science.6828890
Prev | Table of Contents | Next

Articles

Science, Vol 220, Issue 4593, 216-218
Copyright © 1983 by American Association for the Advancement of Science

articles

Formaldehyde damage to DNA and inhibition of DNA repair in human bronchial cells

RC Grafstrom, AJ Fornace Jr, H Autrup, JF Lechner, and CC Harris

Cultured bronchial epithelial and fibroblastic cells from humans were used to study DNA damage and toxicity caused by formaldehyde. Formaldehyde caused the formation of cross-links between DNA and proteins, caused single-strand breaks in DNA, and inhibited the resealing of single-strand breaks produced by ionizing radiation. Formaldehyde also inhibited the unscheduled DNA synthesis that occurs after exposure of cells to ultraviolet irradiation or to benzo[a]pyrene diolexpoxide but at doses substantially higher than those required to inhibit the resealing of x-ray-induced single-strand breaks. Therefore, formaldehyde could exert its mutagenic and carcinogenic effects by both damaging DNA and inhibiting DNA repair.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Clonothrix fusca Roze 1896, a Filamentous, Sheathed, Methanotrophic {gamma}-Proteobacterium.
G. Vigliotta, E. Nutricati, E. Carata, S. M. Tredici, M. De Stefano, P. Pontieri, D. R. Massardo, M. V. Prati, L. De Bellis, and P. Alifano (2007)
Appl. Envir. Microbiol. 73, 3556-3565
   Abstract »    Full Text »    PDF »
Adaptation and acclimatization to formaldehyde in methylotrophs capable of high-concentration formaldehyde detoxification.
R. Chongcharoen, T. J. Smith, K. P. Flint, and H. Dalton (2005)
Microbiology 151, 2615-2622
   Abstract »    Full Text »    PDF »
Formaldehyde Fixation Contributes to Detoxification for Growth of a Nonmethylotroph, Burkholderia cepacia TM1, on Vanillic Acid.
R. Mitsui, Y. Kusano, H. Yurimoto, Y. Sakai, N. Kato, and M. Tanaka (2003)
Appl. Envir. Microbiol. 69, 6128-6132
   Abstract »    Full Text »    PDF »
Physiological role of the glutathione-dependent formaldehyde dehydrogenase in the methylotrophic yeast Candida boidinii.
B. Lee, H. Yurimoto, Y. Sakai, and N. Kato (2002)
Microbiology 148, 2697-2704
   Abstract »    Full Text »    PDF »
A Glutathione-dependent Formaldehyde-activating Enzyme (Gfa) from Paracoccus denitrificans Detected and Purified via Two-dimensional Proton Exchange NMR Spectroscopy.
M. Goenrich, S. Bartoschek, C. H. Hagemeier, C. Griesinger, and J. A. Vorholt (2002)
J. Biol. Chem. 277, 3069-3072
   Abstract »    Full Text »    PDF »
Novel Formaldehyde-Activating Enzyme in Methylobacterium extorquens AM1 Required for Growth on Methanol.
J. A. Vorholt, C. J. Marx, M. E. Lidstrom, and R. K. Thauer (2000)
J. Bacteriol. 182, 6645-6650
   Abstract »    Full Text »    PDF »
Expression of Alcohol Dehydrogenase 3 in Tissue and Cultured Cells from Human Oral Mucosa.
J. J. Hedberg, J.-O. Hoog, J. A. Nilsson, Z. Xi, A. Elfwing, and R. C. Grafstrom (2000)
Am. J. Pathol. 157, 1745-1755
   Abstract »    Full Text »    PDF »
Genotoxicity of formaldehyde in cultured human bronchial fibroblasts.
R. Grafstrom, R. Curren, L. Yang, and C. Harris (1985)
Science 228, 89-91
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)