Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 8 April 1983:
Vol. 220. no. 4593, pp. 214 - 216
DOI: 10.1126/science.6828889
Prev | Table of Contents | Next

Articles

Science, Vol 220, Issue 4593, 214-216
Copyright © 1983 by American Association for the Advancement of Science

articles

Nicotinic cholinergic receptor binding sites in the brain: regulation in vivo

RD Schwartz and KJ Kellar

Tritiated acetylcholine was used to measure binding sites with characteristics of nicotinic cholinergic receptors in rat brain. Regulation of the binding sites in vivo was examined by administering two drugs that stimulate nicotinic receptors directly or indirectly. After 10 days of exposure to the cholinesterase inhibitor diisopropyl fluorophosphate, binding of tritiated acetylcholine in the cerebral cortex was decreased. However, after repeated administration of nicotine for 10 days, binding of tritiated acetylcholine in the cortex was increased. Saturation analysis of tritiated acetylcholine binding in the cortices of rats treated with diisopropyl fluorophosphate or nicotine indicated that the number of binding sites decreased and increased, respectively, while the affinity of the sites was unaltered.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Risk for nicotine dependence and lung cancer is conferred by mRNA expression levels and amino acid change in CHRNA5.
J. C. Wang, C. Cruchaga, N. L. Saccone, S. Bertelsen, P. Liu, J. P. Budde, W. Duan, L. Fox, R. A. Grucza, J. Kern, et al. (2009)
Hum. Mol. Genet. 18, 3125-3135
   Abstract »    Full Text »    PDF »
{beta}2-Nicotinic Acetylcholine Receptor Availability During Acute and Prolonged Abstinence From Tobacco Smoking.
K. P. Cosgrove, J. Batis, F. Bois, P. K. Maciejewski, I. Esterlis, T. Kloczynski, S. Stiklus, S. Krishnan-Sarin, S. O'Malley, E. Perry, et al. (2009)
Arch Gen Psychiatry 66, 666-676
   Abstract »    Full Text »    PDF »
Neurobiology of nicotine dependence.
A. Markou (2008)
Phil Trans R Soc B 363, 3159-3168
   Abstract »    Full Text »    PDF »
Chronic Nicotine Cell Specifically Upregulates Functional {alpha}4* Nicotinic Receptors: Basis for Both Tolerance in Midbrain and Enhanced Long-Term Potentiation in Perforant Path.
R. Nashmi, C. Xiao, P. Deshpande, S. McKinney, S. R. Grady, P. Whiteaker, Q. Huang, T. McClure-Begley, J. M. Lindstrom, C. Labarca, et al. (2007)
J. Neurosci. 27, 8202-8218
   Abstract »    Full Text »    PDF »
Chronic Nicotine Differentially Regulates {alpha}6- and beta3-Containing Nicotinic Cholinergic Receptors in Rat Brain.
D. C. Perry, D. Mao, A. B. Gold, J. M. McIntosh, J. C. Pezzullo, and K. J. Kellar (2007)
J. Pharmacol. Exp. Ther. 322, 306-315
   Abstract »    Full Text »    PDF »
Chronic Nicotine Exposure Enhances Insulin-Induced Mitogenic Signaling via Up-Regulation of {alpha}7 Nicotinic Receptors in Isolated Rat Aortic Smooth Muscle Cells.
T. Wada, M. Naito, H. Kenmochi, H. Tsuneki, and T. Sasaoka (2007)
Endocrinology 148, 790-799
   Abstract »    Full Text »    PDF »
Partial Recovery of Striatal Nicotinic Receptors in 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-Lesioned Monkeys with Chronic Oral Nicotine.
T. Bordia, N. Parameswaran, H. Fan, J. W. Langston, J. M. McIntosh, and M. Quik (2006)
J. Pharmacol. Exp. Ther. 319, 285-292
   Abstract »    Full Text »    PDF »
Sazetidine-A, A Novel Ligand That Desensitizes {alpha}4beta2 Nicotinic Acetylcholine Receptors without Activating Them.
Y. Xiao, H. Fan, J. L. Musachio, Z.-L. Wei, S. K. Chellappan, A. P. Kozikowski, and K. J. Kellar (2006)
Mol. Pharmacol. 70, 1454-1460
   Abstract »    Full Text »    PDF »
Human Tobacco Smokers in Early Abstinence Have Higher Levels of beta2* Nicotinic Acetylcholine Receptors than Nonsmokers..
J. K. Staley, S. Krishnan-Sarin, K. P. Cosgrove, E. Krantzler, E. Frohlich, E. Perry, J. A. Dubin, K. Estok, E. Brenner, R. M. Baldwin, et al. (2006)
J. Neurosci. 26, 8707-8714
   Abstract »    Full Text »    PDF »
Differential Regulation of Mesolimbic {alpha}3/{alpha}6beta2 and {alpha}4beta2 Nicotinic Acetylcholine Receptor Sites and Function after Long-Term Oral Nicotine to Monkeys.
S. E. McCallum, N. Parameswaran, T. Bordia, H. Fan, J. M. McIntosh, and M. Quik (2006)
J. Pharmacol. Exp. Ther. 318, 381-388
   Abstract »    Full Text »    PDF »
Ubiquilin-1 Regulates Nicotine-induced Up-regulation of Neuronal Nicotinic Acetylcholine Receptors.
M. B. Ficklin, S. Zhao, and G. Feng (2005)
J. Biol. Chem. 280, 34088-34095
   Abstract »    Full Text »    PDF »
Exocytic Trafficking Is Required for Nicotine-induced Up-regulation of {alpha}4{beta}2 Nicotinic Acetylcholine Receptors.
T. Darsow, T. K. Booker, J. C. Pina-Crespo, and S. F. Heinemann (2005)
J. Biol. Chem. 280, 18311-18320
   Abstract »    Full Text »    PDF »
Rapid Upregulation of {alpha}7 Nicotinic Acetylcholine Receptors by Tyrosine Dephosphorylation.
C.-H. Cho, W. Song, K. Leitzell, E. Teo, A. D. Meleth, M. W. Quick, and R. A. J. Lester (2005)
J. Neurosci. 25, 3712-3723
   Abstract »    Full Text »    PDF »
Decreased Signs of Nicotine Withdrawal in Mice Null for the {beta}4 Nicotinic Acetylcholine Receptor Subunit.
R. Salas, F. Pieri, and M. De Biasi (2004)
J. Neurosci. 24, 10035-10039
   Abstract »    Full Text »    PDF »
The Comparative Pharmacology and Up-Regulation of Rat Neuronal Nicotinic Receptor Subtype Binding Sites Stably Expressed in Transfected Mammalian Cells.
Y. Xiao and K. J. Kellar (2004)
J. Pharmacol. Exp. Ther. 310, 98-107
   Abstract »    Full Text »    PDF »
Up-Regulation of Brain Nicotinic Acetylcholine Receptors in the Rat during Long-Term Self-Administration of Nicotine: Disproportionate Increase of the {alpha}6 Subunit.
S. L. Parker, Y. Fu, K. McAllen, J. Luo, J. M. McIntosh, J. M. Lindstrom, and B. M. Sharp (2004)
Mol. Pharmacol. 65, 611-622
   Abstract »    Full Text »    PDF »
Subtype-Selective Up-Regulation by Chronic Nicotine of High-Affinity Nicotinic Receptors in Rat Brain Demonstrated by Receptor Autoradiography.
H. N. Nguyen, B. A. Rasmussen, and D. C. Perry (2003)
J. Pharmacol. Exp. Ther. 307, 1090-1097
   Abstract »    Full Text »    PDF »
Differential Regulation of Nicotinic Acetylcholine Receptors in PC12 Cells by Nicotine and Nerve Growth Factor.
A. M. Avila, M. I. Davila-Garcia, V. S. Ascarrunz, Y. Xiao, and K. J. Kellar (2003)
Mol. Pharmacol. 64, 974-986
   Abstract »    Full Text »    PDF »
Pathophysiological Effects of Nicotine on the Pancreas: An Update.
P. Chowdhury, S. MacLeod, K. B. Udupa, and P. L. Rayford (2002)
Experimental Biology and Medicine 227, 445-454
   Abstract »    Full Text »    PDF »
Agonist Regulation of Rat alpha 3beta 4 Nicotinic Acetylcholine Receptors Stably Expressed in Human Embryonic Kidney 293 Cells.
E. L. Meyer, Y. Xiao, and K. J. Kellar (2001)
Mol. Pharmacol. 60, 568-576
   Abstract »    Full Text »    PDF »
Desensitisation of chromaffin cell nicotinic receptors does not impede catecholamine secretion during acute hypoxia in rainbow trout (Oncorhynchus mykiss).
K. Lapner, C. Montpetit, and S. Perry (2000)
J. Exp. Biol. 203, 1589-1597
   Abstract »    PDF »
Cigarette Smoking.
A. W. Bergen and N. Caporaso (1999)
J Natl Cancer Inst 91, 1365-1375
   Abstract »    Full Text »    PDF »
Increased Nicotinic Receptors in Brains from Smokers: Membrane Binding and Autoradiography Studies.
D. C. Perry, M. I. Dávila-García, C. A. Stockmeier, and K. J. Kellar (1999)
J. Pharmacol. Exp. Ther. 289, 1545-1552
   Abstract »    Full Text »
Effect of Smoking History on [3H]Nicotine Binding in Human Postmortem Brain.
C. R. Breese, M. J. Marks, J. Logel, C. E. Adams, B. Sullivan, A. C. Collins, and S. Leonard (1997)
J. Pharmacol. Exp. Ther. 282, 7-13
   Abstract »    Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)