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Science 18 March 1983:
Vol. 219. no. 4590, pp. 1343 - 1345
DOI: 10.1126/science.6828863
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Articles

Science, Vol 219, Issue 4590, 1343-1345
Copyright © 1983 by American Association for the Advancement of Science

articles

Collagen formation by the hepatocyte in primary monolayer culture and in vivo

RF Diegelmann, PS Guzelian, R Gay, and S Gay

Immunohistochemical techniques were used to confirm biochemical evidence that parenchymal cells isolated from adult rat liver and maintained in nonreplicating monolayer culture for 2 days synthesized type IV basement membrane collagen. On continued incubation in serum-free medium, the hepatocytes also synthesized the interstitial collagens, types I and III. Consistent with these results in culture, type IV collagen was localized to the hepatocytes in slices of pathologic rat liver. Hence collagen formation is a previously unrecognized function of the hepatocyte that may be important in the pathogenesis of liver fibrosis or cirrhosis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Capillarization of Hepatic Sinusoid by Liver Endothelial Cell-Reactive Autoantibodies in Patients with Cirrhosis and Chronic Hepatitis.
B. Xu, U. Broome, M. Uzunel, S. Nava, X. Ge, M. Kumagai-Braesch, K. Hultenby, B. Christensson, B.-G. Ericzon, J. Holgersson, et al. (2003)
Am. J. Pathol. 163, 1275-1289
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