Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 18 March 1983:
Vol. 219. no. 4590, pp. 1335 - 1337
DOI: 10.1126/science.6828861
Prev | Table of Contents | Next

Articles

Science, Vol 219, Issue 4590, 1335-1337
Copyright © 1983 by American Association for the Advancement of Science

articles

Increased sensitivity of lymphocytes from people over 65 to cell cycle arrest and chromosomal damage

L Staiano-Coico, Z Darzynkiewicz, JM Hefton, R Dutkowski, GJ Darlington, and ME Weksler

Flow cytometry revealed that, in the presence of tritiated thymidine, a greater percentage of phytohemagglutinin-stimulated lymphocytes from old human donors were arrested in the G2 or M phase than were cells from young donors. Furthermore, lymphocytes from old donors showed significantly more chromosomal damage than did lymphocytes from young donors. Lymphocyte cultures from old or young donors not exposed to tritiated thymidine had the same percentage of cycling lymphocytes in G2 or M, although the number of lymphocytes stimulated by phytohemagglutinin to enter the cell cycle was significantly lower in cultures from old donors. Thus, the impaired incorporation of tritiated thymidine by phytohemagglutinin-exposed lymphocytes from old humans reflects both an impaired proliferative response to phytohemagglutinin and an increased sensitivity to the radiobiological effects of tritiated thymidine.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Age-Associated Decline in cdk1 Activity Delays Cell Cycle Progression of Human T Lymphocytes.
R. A. Quadri, A. Arbogast, M.-A. Phelouzat, S. Boutet, O. Plastre, and J. J. Proust (1998)
J. Immunol. 161, 5203-5209
   Abstract »    Full Text »    PDF »
Cancer in the Elderly: Basic Science and Clinical Aspects.
D. A. LIPSCHITZ, S. GOLDSTEIN, R. REIS, M. E. WEKSLER, R. BRESSLER, and B. A. NEILAN (1985)
Ann Intern Med 102, 218-228
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)