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Science 11 March 1983:
Vol. 219. no. 4589, pp. 1228 - 1230
DOI: 10.1126/science.6402815
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Articles

Science, Vol 219, Issue 4589, 1228-1230
Copyright © 1983 by American Association for the Advancement of Science

articles

Stable antibody-producing murine hybridomas

RT Taggart and IM Samloff

A method is described for obtaining antibody-producing hybridomas that are preferentially retained in cultures of fused mouse spleen and myeloma cells. Hybridomas are produced by fusing mouse myeloma cells that are deficient in adenosine phosphoribosyltransferase (APRT) with mouse spleen cells containing Robertsonian 8.12 translocation chromosomes. The cell fusion mixtures are exposed to a culture medium that can be utilized only by APRT-positive cells, which results in the elimination of both unfused APRT-deficient myeloma cells and non-antibody-producing APRT-deficient hybridomas that arise by segregation of the 8.12 translocation chromosomes containing the APRT genes and the active heavy chain immunoglobulin gene.


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