Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 11 March 1983:
Vol. 219. no. 4589, pp. 1225 - 1228
DOI: 10.1126/science.6298935
Prev | Table of Contents | Next

Articles

Science, Vol 219, Issue 4589, 1225-1228
Copyright © 1983 by American Association for the Advancement of Science

articles

Infection of normal human epithelial cells by Epstein-Barr virus

IM Shapiro and DJ Volsky

Primary cultures of epithelial cells were grown from the tonsils and adenoids of patients with diseases not related to Epstein-Barr virus. The cells could not be infected by Epstein-Barr virus. Fluorescein-labeled Epstein-Barr virus and a cytofluorograph were then used to show that the epithelial cells do not have detectable receptors for the virus. However, implantation with Epstein-Barr virus receptors gave the cells the ability to bind the labeled virus. One to 5 percent of receptor-implanted cells exposed to the transforming B95-8 substrain of the virus expressed Epstein-Barr nuclear antigen. The early and viral capsid Epstein-Barr virus-determined antigens were not detected in the virus-infected cultures. The results show that normal human epithelial cells from the nasopharynx become susceptible to infection by Epstein-Barr virus when the membrane barrier resulting from the lack of viral receptors is overcome by receptor implantation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Ex Vivo Modeling of Oral HIV Transmission in Human Palatine Tonsil.
D. M. Maher, Z.-Q. Zhang, T. W. Schacker, and P. J. Southern (2005)
J. Histochem. Cytochem. 53, 631-642
   Abstract »    Full Text »    PDF »
Epstein-Barr Virus Infection in Ex Vivo Tonsil Epithelial Cell Cultures of Asymptomatic Carriers.
D. M. Pegtel, J. Middeldorp, and D. A. Thorley-Lawson (2004)
J. Virol. 78, 12613-12624
   Abstract »    Full Text »    PDF »
Infection of intestinal epithelial cells and development of systemic disease following gastric instillation of murine gammaherpesvirus-68.
J. W. Peacock and K. L. Bost (2000)
J. Gen. Virol. 81, 421-429
   Abstract »    Full Text »
Cell Fusion by Nasopharyngeal Carcinoma-Derived Epstein-Barr Virus.
T. Takimoto, H. Sato, H. Ogura, and T. Miyazaki (1987)
Arch Otolaryngol Head Neck Surg 113, 510-513
   Abstract »    PDF »
Primary Sjogren's Syndrome After Infectious Mononucleosis.
S. WHITTINGHAM, J. McNEILAGE, and I. R. MACKAY (1985)
Ann Intern Med 102, 490-493
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)