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Science 18 February 1983:
Vol. 219. no. 4586, pp. 867 - 869
DOI: 10.1126/science.6681677
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Articles

Science, Vol 219, Issue 4586, 867-869
Copyright © 1983 by American Association for the Advancement of Science

articles

Actin filament stress fibers in vascular endothelial cells in vivo

AJ Wong, TD Pollard, and IM Herman

Fluorescence microscopy with 7-nitrobenz-2-oxa-3-diazole phallacidin was used to survey vertebrate tissues for actin filament bundles comparable to the stress fibers of cultured cells. Such bundles were found only in vascular endothelial cells. Like the stress fibers of cultured cells, these actin filament bundles were stained in a punctate pattern by fluorescent antibodies to both alpha-actinin and myosin. The stress fibers were oriented parallel to the direction of blood flow and were prominent in endothelial cells from regions exposed to high-velocity flow, such as the left ventricle, aortic valve, and aorta. Actin bundles may help the endothelial cell to withstand hemodynamic stress.


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