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Science 18 February 1983:
Vol. 219. no. 4586, pp. 853 - 856
DOI: 10.1126/science.6337398
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Articles

Science, Vol 219, Issue 4586, 853-856
Copyright © 1983 by American Association for the Advancement of Science

articles

The human c-ras1H oncogene: a mutation in normal and neoplastic tissue from the same patient

RJ Muschel, G Khoury, P Lebowitz, R Koller, and R Dhar

The c-ras1H oncogene can be distinguished from its normal cellular counterpart by the loss of a restriction endonuclease site. This sequence alteration is the basis of a rapid screening method for the presence of this oncogene. DNA's from 34 individuals were screened by this method, and all were homozygous for the normal allele. In contrast, DNA from a patient's bladder tumor, as well as DNA from his normal bladder and leukocytes, were heterozygous at that restriction endonuclease site. Further restriction enzyme mapping pinpointed the change in the mutant allele as being one of two nucleotides, either of which would change the 12th amino acid (glycine) in the normal c-ras1H gene product. Point mutations in the codon for this amino acid have previously been described in a bladder tumor cell line and in the viral oncogene v-rasH. These results indicate that the patient carried a c-ras1H oncogene in his germ line, raising the possibility that the c-ras1H oncogene confers a predisposition to neoplasia.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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S. Needleman, Y Yuasa, S Srivastava, and S. Aaronson (1983)
Science 222, 173-175
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Mutation affecting the 12th amino acid of the c-Ha-ras oncogene product occurs infrequently in human cancer.
A. Feinberg, B Vogelstein, M. Droller, S. Baylin, and B. Nelkin (1983)
Science 220, 1175-1177
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Retraction of data on the human c-ras1H oncogene.
R. Muschel, G Khoury, P Lebowitz, R Koller, and R Dhar (1983)
Science 220, 336
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