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Science 18 February 1983:
Vol. 219. no. 4586, pp. 845 - 847
DOI: 10.1126/science.6823553
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Articles

Science, Vol 219, Issue 4586, 845-847
Copyright © 1983 by American Association for the Advancement of Science

articles

Attogram detection limit for aqueous dye samples by laser-induced fluorescence

NJ Dovichi, JC Martin, JH Jett, and RA Keller

A modified flow cytometer has been used to detect attogram quantities of aqueous rhodamine 6G by laser-induced fluorescence analysis. A detection limit of 28 attograms (35,000 molecules) was obtained, nearly two orders of magnitude better than earlier measurements. The detection limit in concentration units was 1.4 x 10(-13) mole per liter. During the 1-second measurement period, the total volume sampled was 0.42 microliter. On average, only half a rhodamine 6G molecule was present in the 6-picoliter probed volume.


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