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Science 11 February 1983:
Vol. 219. no. 4585, pp. 620 - 625
DOI: 10.1126/science.6186023
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Articles

Science, Vol 219, Issue 4585, 620-625
Copyright © 1983 by American Association for the Advancement of Science

articles

Secretion of human interferons by yeast

RA Hitzeman, DW Leung, LJ Perry, WJ Kohr, HL Levine, and DV Goeddel

Plasmids were constructed to direct synthesis of the human interferons IFN-alpha 1, IFN-alpha 2, and IFN-gamma in the yeast Saccharomyces cerevisiae. Expression of IFN genes containing coding sequences for secretion signals resulted in the secretion of IFN activity. A large proportion of the IFN-alpha 1 and IFN-alpha 2 isolated from the yeast cell growth media had the same amino termini as the natural mature interferons, suggesting a removal of the signal sequences identical to that of human cells. These results show that a lower eukaryote, such as yeast, can utilize and process a human signal sequence.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Engineering of Polyploid Saccharomyces cerevisiae for Secretion of Large Amounts of Fungal Glucoamylase.
K. Ekino, H. Hayashi, M. Moriyama, M. Matsuda, M. Goto, S. Yoshino, and K. Furukawa (2002)
Appl. Envir. Microbiol. 68, 5693-5697
   Abstract »    Full Text »    PDF »
Ouabain and substrate affinities of human Na+-K+-ATPase alpha 1beta 1, alpha 2beta 1, and alpha 3beta 1 when expressed separately in yeast cells.
J. Muller-Ehmsen, P. Juvvadi, C. B. Thompson, L. Tumyan, M. Croyle, J. B. Lingrel, R. H. G. Schwinger, A. A. McDonough, and R. A. Farley (2001)
Am J Physiol Cell Physiol 281, C1355-C1364
   Abstract »    Full Text »    PDF »
Mechanistic Studies on Thiaminase I.
C. A. Costello, N. L. Kelleher, M. Abe, F. W. McLafferty, and T. P. Begley (1996)
J. Biol. Chem. 271, 3445-3452
   Abstract »    Full Text »    PDF »
Golgi Localization in Yeast Is Mediated by the Membrane Anchor Region of Rat Liver Sialyltransferase.
T. Schwientek, C. Lorenz, and J. F. Ernst (1995)
J. Biol. Chem. 270, 5483-5489
   Abstract »    Full Text »    PDF »


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