Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 11 February 1983:
Vol. 219. no. 4585, pp. 614 - 620
DOI: 10.1126/science.6297004
Prev | Table of Contents | Next

Articles

Science, Vol 219, Issue 4585, 614-620
Copyright © 1983 by American Association for the Advancement of Science

articles

Rabies virus glycoprotein analogs: biosynthesis in Escherichia coli

E Yelverton, S Norton, JF Obijeski, and DV Goeddel

The surface of rabies virus is composed of an approximately 60,000 dalton glycoprotein, in which most of the antigenic and immunogenic determinants of the virus reside. We have constructed plasmids for the direct expression in Escherichia coli of the mature full length rabies glycoprotein gene and also for the expression of a glycoprotein gene which has been truncated to exclude the coding region for a hydrophobic, possibly transmembrane, domain of the protein. Escherichia coli harboring the plasmids synthesize analog proteins which conform by several biochemical and antigenic criteria to rabies glycoprotein.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mutations Conferring Resistance to Neutralization by a Soluble Form of the Neurotrophin Receptor (p75NTR) Map outside of the Known Antigenic Sites of the Rabies Virus Glycoprotein.
C. Langevin and C. Tuffereau (2002)
J. Virol. 76, 10756-10765
   Abstract »    Full Text »    PDF »
An Avirulent Mutant of Rabies Virus Is Unable To Infect Motoneurons In Vivo and In Vitro.
P. Coulon, J.-P. Ternaux, A. Flamand, and C. Tuffereau (1998)
J. Virol. 72, 273-278
   Abstract »    Full Text »    PDF »
The Hydroxy Amino Acid in an Asn-X-Ser/Thr Sequon Can Influence N-Linked Core Glycosylation Efficiency and the Level of Expression of a Cell Surface Glycoprotein.
L. Kasturi, J. R. Eshleman, W. H. Wunner, and S. H. Shakin-Eshleman (1995)
J. Biol. Chem. 270, 14756-14761
   Abstract »    Full Text »    PDF »
Amino acid sequence similarity between rabies virus glycoprotein and snake venom curaremimetic neurotoxins.
T. Lentz, P. Wilson, E Hawrot, and D. Speicher (1984)
Science 226, 847-848
   Abstract »    PDF »
Detection of antibodies to herpes simplex virus with a continuous cell line expressing cloned glycoprotein D.
P. Berman, D Dowbenko, L. Lasky, and C. Simonsen (1983)
Science 222, 524-527
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)