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Science 28 January 1983:
Vol. 219. no. 4583, pp. 395 - 397
DOI: 10.1126/science.6294832
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Articles

Science, Vol 219, Issue 4583, 395-397
Copyright © 1983 by American Association for the Advancement of Science

articles

Chromosome localization of highly repetitive human DNA's and amplified ribosomal DNA with restriction enzymes

DA Miller, YC Choi, and OJ Miller

Restriction endonucleases cut and partially removed DNA throughout fixed air-dried human metaphase chromosomes. Some enzymes produced a G-banding pattern; some revealed the presence of multiple chromosome-specific classes of highly repetitive DNA in C-band heterochromatin. Enzymes that produced the informative C-band patterns had recognition sequences that were four or five, but not six, base pairs long and did not contain a cytosine-guanine doublet. In both rat and human chromosomes, regions containing amplified ribosomal RNA genes were specifically removed by the restriction endonuclease Msp I.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)