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Science 21 January 1983:
Vol. 219. no. 4582, pp. 299 - 301
DOI: 10.1126/science.6849137
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Articles

Science, Vol 219, Issue 4582, 299-301
Copyright © 1983 by American Association for the Advancement of Science

articles

Insulin receptor: evidence that it is a protein kinase

RA Roth and DJ Cassell

Highly purified preparations of insulin receptor catalyzed the phosphorylation of the 95,000-dalton subunit of the insulin receptor. This subunit of the insulin receptor was also labeled with [alpha-32P]8-azidoadenosine 5'-triphosphate, a photoaffinity label for adenosine triphosphate binding sites. The identity of the 95,000-dalton band was confirmed in both cases by precipitation with a monoclonal antibody to the insulin receptor. These results suggest that the insulin receptor is itself a protein kinase.


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