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Science 14 January 1983:
Vol. 219. no. 4581, pp. 174 - 176
DOI: 10.1126/science.6294829
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Articles

Science, Vol 219, Issue 4581, 174-176
Copyright © 1983 by American Association for the Advancement of Science

articles

Recombination during gene transfer into mouse cells can restore the function of deleted genes

J Small and G Scangos

Two plasmids containing nonoverlapping deletions of the herpes simplex virus thymidine kinase gene were introduced into thymidine kinase-deficient mouse L cells by DNA-mediated gene transfer. Thymidine kinase-producing transformants were generated by a mixture of the two plasmids at a frequency significantly greater than that generated by either plasmid alone. Southern blot analyses demonstrated that functional thymidine kinase genes were generated by homologous recombination between the two deletion mutants.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Presence of Replicating Virus in Recombinant Hepadnavirus Stocks Results from Recombination and Can Be Eliminated by the Use of a Packaging Cell Line.
U. Klocker, H. Oberwinkler, T. Kurschner, and U. Protzer (2003)
J. Virol. 77, 2873-2881
   Abstract »    Full Text »    PDF »
Gene transfer and molecular cloning of the human NGF receptor.
M. Chao, M. Bothwell, A. Ross, H Koprowski, A. Lanahan, C. Buck, and A Sehgal (1986)
Science 232, 518-521
   Abstract »    PDF »
Analysis of Homologous Recombination in Cultured Mammalian Cells.
K. Folger, K. Thomas, and M.R. Capecchi (1984)
Cold Spring Harb Symp Quant Biol 49, 123-138
   Abstract »    PDF »
Homologous Recombination in Mouse L Cells.
F.-L. Lin, K. Sperle, and N. Sternberg (1984)
Cold Spring Harb Symp Quant Biol 49, 139-149
   Abstract »    PDF »
The Recombination and Integration of DNAs Introduced into Mouse L Cells.
D.A. Brenner, S. Kato, R.A. Anderson, A.C. Smigocki, and R.D. Camerini-Otero (1984)
Cold Spring Harb Symp Quant Biol 49, 151-160
   Abstract »    PDF »
Homologous Recombination with DNA Introduced into Mammalian Cells.
O. Smithies, M.A. Koralewski, K.-Y. Song, and R.S. Kucherlapati (1984)
Cold Spring Harb Symp Quant Biol 49, 161-170
   Abstract »    PDF »
Homologous Recombination between Repeated Chromosomal Sequences in Mouse Cells.
R.M. Liskay, J.L. Stachelek, and A. Letsou (1984)
Cold Spring Harb Symp Quant Biol 49, 183-189
   Abstract »    PDF »
Homologous Recombination in Monkey Cells and Human Cell-free Extracts.
R.S. Kucherlapati, D. Ayares, A. Hanneken, K. Noonan, S. Rauth, J.M. Spencer, L. Wallace, and P.D. Moore (1984)
Cold Spring Harb Symp Quant Biol 49, 191-197
   Abstract »    PDF »


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