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Science 17 December 1982:
Vol. 218. no. 4578, pp. 1219 - 1221
DOI: 10.1126/science.6293054
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Articles

Science, Vol 218, Issue 4578, 1219-1221
Copyright © 1982 by American Association for the Advancement of Science

articles

Crypts are the site of intestinal fluid and electrolyte secretion

MJ Welsh, PL Smith, M Fromm, and RA Frizzell

The site of adenosine 3',5'-monophosphate-mediated fluid and electrolyte secretion across mammalian large intestine was found to be the crypts of Lieberkuhn by means of two techniques. First, the formation of fluid droplets was visualized on the oil-covered mucosal surface directly over crypt duct openings when secretion was stimulated. Second, microelectrode impalement of individual surface and crypt cells revealed that only crypts cells produced a pattern of secretagogue induced alterations in membrane potential and resistance that was characteristic of secretory epithelia.


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