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Science 10 December 1982:
Vol. 218. no. 4577, pp. 1131 - 1133
DOI: 10.1126/science.6293053
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Articles

Science, Vol 218, Issue 4577, 1131-1133
Copyright © 1982 by American Association for the Advancement of Science

articles

In vivo identification of the transforming gene product of simian sarcoma virus

KC Robbins, SG Devare, EP Reddy, and SA Aaronson

Simian sarcoma virus (SSV) deletion mutants were constructed from a molecular clone containing the entire infectious provirus. Transfection analysis of these mutants localized the SSV transforming gene to a small region of the viral genome encompassing its cell-derived sequence (v-sis). Antiserum to a peptide synthesized on the basis of the predicted amino acid sequence of the SSV transforming gene detected a 28,000-dalton protein that was specifically expressed in SSV transformed cells and that corresponded in size to that predicted from the v-sis coding sequence. The v-sis gene product designated p28sis was not a phosphoprotein, nor did it possess detectable protein kinase activity. These findings distinguish p28sis from a number of other retroviral onc proteins.


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