Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 10 December 1982:
Vol. 218. no. 4577, pp. 1131 - 1133
DOI: 10.1126/science.6293053
Prev | Table of Contents | Next

Articles

Science, Vol 218, Issue 4577, 1131-1133
Copyright © 1982 by American Association for the Advancement of Science

articles

In vivo identification of the transforming gene product of simian sarcoma virus

KC Robbins, SG Devare, EP Reddy, and SA Aaronson

Simian sarcoma virus (SSV) deletion mutants were constructed from a molecular clone containing the entire infectious provirus. Transfection analysis of these mutants localized the SSV transforming gene to a small region of the viral genome encompassing its cell-derived sequence (v-sis). Antiserum to a peptide synthesized on the basis of the predicted amino acid sequence of the SSV transforming gene detected a 28,000-dalton protein that was specifically expressed in SSV transformed cells and that corresponded in size to that predicted from the v-sis coding sequence. The v-sis gene product designated p28sis was not a phosphoprotein, nor did it possess detectable protein kinase activity. These findings distinguish p28sis from a number of other retroviral onc proteins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Platelet-derived Growth Factor D: Tumorigenicity in Mice and Dysregulated Expression in Human Cancer.
W. J. LaRochelle, M. Jeffers, J. R. F. Corvalan, X.-C. Jia, X. Feng, S. Vanegas, J. D. Vickroy, X.-D. Yang, F. Chen, G. Gazit, et al. (2002)
Cancer Res. 62, 2468-2473
   Abstract »    Full Text »    PDF »
Transcriptional Regulation of the SIS/PDGF-B Gene in Human Osteosarcoma Cells by the Sp Family of Transcription Factors.
Y. Liang, D. F. Robinson, Jör. Dennig, G. Suske, and W. E. Fahl (1996)
J. Biol. Chem. 271, 11792-11797
   Abstract »    Full Text »    PDF »
A novel mechanism regulating growth factor association with the cell surface: identification of a PDGF retention domain..
W J LaRochelle, M May-Siroff, K C Robbins, and S A Aaronson (1991)
Genes & Dev. 5, 1191-1199
   Abstract »    PDF »
Expression of gene rrg is associated with reversion of NIH 3T3 transformed by LTR-c-H-ras.
S Contente, K Kenyon, D Rimoldi, and R. Friedman (1990)
Science 249, 796-798
   Abstract »    PDF »
Transformation by v-sis occurs by an internal autoactivation mechanism.
B. Bejcek, D. Li, and T. Deuel (1989)
Science 245, 1496-1499
   Abstract »    PDF »
Comparison of biological properties and transforming potential of human PDGF-A and PDGF-B chains.
M. Beckmann, C Betsholtz, C. Heldin, B Westermark, E Di Marco, P. Di Fiore, K. Robbins, and S. Aaronson (1988)
Science 241, 1346-1349
   Abstract »    PDF »
Autocrine stimulation of intracellular PDGF receptors in v-sis-transformed cells.
M. Keating and L. Williams (1988)
Science 239, 914-916
   Abstract »    PDF »
Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells.
D. Graves, A. Owen, R. Barth, P Tempst, A Winoto, L Fors, L. Hood, and H. Antoniades (1984)
Science 226, 972-974
   Abstract »    PDF »
Transforming potential of human c-sis nucleotide sequences encoding platelet-derived growth factor.
S. Josephs, L Ratner, M. Clarke, E. Westin, M. Reitz, and F Wong-Staal (1984)
Science 225, 636-639
   Abstract »    PDF »
Oncogenes: Implications for the Diagnosis and Treatment of Cancer.
M. J. CLINE, D. J. SLAMON, and J. S. LIPSICK (1984)
Ann Intern Med 101, 223-233
   Abstract »    PDF »
Simian sarcoma virus--transformed cells secrete a mitogen identical to platelet-derived growth factor.
A. Owen, P Pantazis, and H. Antoniades (1984)
Science 225, 54-56
   Abstract »    PDF »
Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor.
R. Doolittle, M. Hunkapiller, L. Hood, S. Devare, K. Robbins, S. Aaronson, and H. Antoniades (1983)
Science 221, 275-277
   Abstract »    PDF »
Antibodies that react with predetermined sites on proteins.
J. Sutcliffe, T. Shinnick, N Green, and R. Lerner (1983)
Science 219, 660-666
   Abstract »    PDF »
NM23-H1 and NM23-H2 Repress Transcriptional Activities of Nuclease-hypersensitive Elements in the Platelet-derived Growth Factor-A Promoter.
D. Ma, Z. Xing, B. Liu, N. G. Pedigo, S. G. Zimmer, Z. Bai, E. H. Postel, and D. M. Kaetzel (2002)
J. Biol. Chem. 277, 1560-1567
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)