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Science 29 October 1982:
Vol. 218. no. 4571, pp. 474 - 475
DOI: 10.1126/science.7123245

Articles

Science, Vol 218, Issue 4571, 474-475
Copyright © 1982 by American Association for the Advancement of Science


articles

Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication

HG Gratzner

Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro. The immunoglobulin-producing hybridomas were derived from spleen cells of mice immunized with a conjugate of iodouridine and ovalbumin. The cells were fused with the plasmacytoma line SP2/0Ag14. The antibodies produced are highly specific for bromodeoxyuridine and iododeoxyuridine and do not cross-react with thymidine. DNA synthesis in cultured cells exposed to bromodeoxyuridine for as short a time as 6 minutes can be detected easily and rapidly by an immunofluorescent staining method and quantitated by flow cytometry.


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Am J Physiol Gastrointest Liver Physiol 274, G406-G412
   Abstract »    Full Text »    PDF »
Measurement of cell proliferation by labeling of DNA with stable isotope-labeled glucose: Studies in vitro, in animals, and in humans.
D. C. Macallan, C. A. Fullerton, R. A. Neese, K. Haddock, S. S. Park, and M. K. Hellerstein (1998)
PNAS 95, 708-713
   Abstract »    Full Text »    PDF »
Basal ganglia precursors found in aggregates following embryonic transplantation adopt a striatal phenotype in heterotopic locations.
L Magrassi, M. Ehrlich, G Butti, S Pezzotta, S Govoni, and E Cattaneo (1998)
Development 125, 2847-2855
   Abstract »    PDF »
Diminished Sonic hedgehog signaling and lack of floor plate differentiation in Gli2 mutant mice.
Q Ding, J Motoyama, S Gasca, R Mo, H Sasaki, J Rossant, and C. Hui (1998)
Development 125, 2533-2543
   Abstract »    PDF »
Ecdysone pathway is required for furrow progression in the developing Drosophila eye.
C. Brennan, M Ashburner, and K Moses (1998)
Development 125, 2653-2664
   Abstract »    PDF »
Ecdysteroids govern two phases of eye development during metamorphosis of the moth, Manduca sexta.
D. Champlin and J. Truman (1998)
Development 125, 2009-2018
   Abstract »    PDF »
Ecdysteroid control of cell proliferation during optic lobe neurogenesis in the moth Manduca sexta.
D. Champlin and J. Truman (1998)
Development 125, 269-277
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Hindlimb unloading in rat decreases preosteoblast proliferation assessed in vivo with BrdU incorporation.
O. Barou, S. Palle, L. Vico, C. Alexandre, and M.-H. Lafage-Proust (1998)
Am J Physiol Endocrinol Metab 274, E108-E114
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Expression Patterns of Developmental Control Genes in Normal and Engrailed-1 Mutant Mouse Spinal Cord Reveal Early Diversity in Developing Interneurons.
M. P. Matise and A. L. Joyner (1997)
J. Neurosci. 17, 7805-7816
   Abstract »    Full Text »    PDF »
Olfactory Bulb Recovery after Early Sensory Deprivation.
D. M. Cummings, H. E. Henning, and P. C. Brunjes (1997)
J. Neurosci. 17, 7433-7440
   Abstract »    Full Text »    PDF »
Immunohistochemical Analysis of DNA Synthesis During Chronic Stimulation with Isoproterenol in Mouse Submandibular Gland.
S. Matsuura and K. Suzuki (1997)
J. Histochem. Cytochem. 45, 1137-1146
   Abstract »    Full Text »    PDF »
Protease Antigen Recovery Decreases the Specificity of Bromodeoxyuridine Detection In Formalin-fixed Tissue.
P. M. Bak and R. J. Panos (1997)
J. Histochem. Cytochem. 45, 1165-1168
   Abstract »    Full Text »    PDF »
CD44 Is Involved in Tumor Angiogenesis; an Activation Antigen on Human Endothelial Cells.
A. W. Griffioen, M. J.H. Coenen, C. A. Damen, S. M.M. Hellwig, D. H.J. van Weering, W. Vooys, G. H. Blijham, and G. Groenewegen (1997)
Blood 90, 1150-1159
   Abstract »    Full Text »    PDF »
Expression and activation of SH2/PTB-containing ShcA adaptor protein reflects the pattern of neurogenesis in the mammalian brain.
L. Conti, C. De Fraja, M. Gulisano, E. Migliaccio, S. Govoni, and E. Cattaneo (1997)
PNAS 94, 8185-8190
   Abstract »    Full Text »    PDF »



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