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Science 29 October 1982: Vol. 218. no. 4571, pp. 474 - 475 DOI: 10.1126/science.7123245
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Articles
Science, Vol 218, Issue 4571, 474-475
Copyright © 1982 by American Association for the Advancement of Science
Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication
HG Gratzner
Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro. The immunoglobulin-producing hybridomas were derived from spleen cells of mice immunized with a conjugate of iodouridine and ovalbumin. The cells were fused with the plasmacytoma line SP2/0Ag14. The antibodies produced are highly specific for bromodeoxyuridine and iododeoxyuridine and do not cross-react with thymidine. DNA synthesis in cultured cells exposed to bromodeoxyuridine for as short a time as 6 minutes can be detected easily and rapidly by an immunofluorescent staining method and quantitated by flow cytometry.
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PNAS
95, 10878-10883
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- Reduced susceptibility of mice overexpressing transforming growth factor alpha to dextran sodium sulphate induced colitis.
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Gut
43, 64-70
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- Allergen Challenge Increases Cell Traffic between Bone Marrow and Lung.
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Am. J. Respir. Cell Mol. Biol.
18, 759-767
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- Repeated allergen inhalations induce DNA synthesis in airway smooth muscle and epithelial cells in vivo.
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Am J Physiol Lung Cell Mol Physiol
274, L417-L424
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- CCK-, secretin-, and cholinergic-independent pancreatic fluid hypersecretion in protease inhibitor-treated rats.
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Am J Physiol Gastrointest Liver Physiol
274, G406-G412
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- Measurement of cell proliferation by labeling of DNA with stable isotope-labeled glucose: Studies in vitro, in animals, and in humans.
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PNAS
95, 708-713
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- Basal ganglia precursors found in aggregates following embryonic transplantation adopt a striatal phenotype in heterotopic locations.
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Development
125, 2847-2855
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- Diminished Sonic hedgehog signaling and lack of floor plate differentiation in Gli2 mutant mice.
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Development
125, 2533-2543
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- Ecdysone pathway is required for furrow progression in the developing Drosophila eye.
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Development
125, 2653-2664
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- Ecdysteroids govern two phases of eye development during metamorphosis of the moth, Manduca sexta.
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Development
125, 2009-2018
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- Ecdysteroid control of cell proliferation during optic lobe neurogenesis in the moth Manduca sexta.
- D. Champlin and J. Truman (1998)
Development
125, 269-277
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- Hindlimb unloading in rat decreases preosteoblast proliferation assessed in vivo with BrdU incorporation.
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Am J Physiol Endocrinol Metab
274, E108-E114
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- Expression Patterns of Developmental Control Genes in Normal and Engrailed-1 Mutant Mouse Spinal Cord Reveal Early Diversity in Developing Interneurons.
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J. Neurosci.
17, 7805-7816
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- Olfactory Bulb Recovery after Early Sensory Deprivation.
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- Immunohistochemical Analysis of DNA Synthesis During Chronic Stimulation with Isoproterenol in Mouse Submandibular Gland.
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J. Histochem. Cytochem.
45, 1137-1146
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- Protease Antigen Recovery Decreases the Specificity of Bromodeoxyuridine Detection In Formalin-fixed Tissue.
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J. Histochem. Cytochem.
45, 1165-1168
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- CD44 Is Involved in Tumor Angiogenesis; an Activation Antigen on Human Endothelial Cells.
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Blood
90, 1150-1159
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- Expression and activation of SH2/PTB-containing ShcA adaptor protein reflects the pattern of neurogenesis in the mammalian brain.
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PNAS
94, 8185-8190
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- Paclitaxel Inhibits Arterial Smooth Muscle Cell Proliferation and Migration In Vitro and In Vivo Using Local Drug Delivery.
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Circulation
96, 636-645
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