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Science 22 October 1982: Vol. 218. no. 4570, pp. 348 - 353 DOI: 10.1126/science.6289436
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Articles
Science, Vol 218, Issue 4570, 348-353
Copyright © 1982 by American Association for the Advancement of Science
Genetic transformation of Drosophila with transposable element vectors
GM Rubin
and
AC Spradling
Exogenous DNA sequences were introduced into the Drosophila germ line. A rosy transposon (ry1), constructed by inserting a chromosomal DNA fragment containing the wild-type rosy gene into a P transposable element, transformed germ line cells in 20 to 50 percent of the injected rosy mutant embryos. Transformants contained one or two copies of chromosomally integrated, intact ry1 that were stably inherited in subsequent generations. These transformed flies had wild-type eye color indicating that the visible genetic defect in the host strain could be fully and permanently corrected by the transferred gene. To demonstrate the generality of this approach, a DNA segment that does not confer a recognizable phenotype on recipients was also transferred into germ line chromosomes.
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- Drosophila Reptin and Other TIP60 Complex Components Promote Generation of Silent Chromatin.
- D. Qi, H. Jin, T. Lilja, and M. Mannervik (2006)
Genetics
174, 241-251
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- Of Two Cytosolic Aconitases Expressed in Drosophila, Only One Functions as an Iron-regulatory Protein.
- M. I. Lind, F. Missirlis, O. Melefors, H. Uhrigshardt, K. Kirby, J. P. Phillips, K. Soderhall, and T. A. Rouault (2006)
J. Biol. Chem.
281, 18707-18714
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- Characterization of Drosophila mini-me, a Gene Required for Cell Proliferation and Survival.
- C. Jones, R. Reifegerste, and K. Moses (2006)
Genetics
173, 793-808
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- Site-Specific Transformation of Drosophila via {phi}C31 Integrase-Mediated Cassette Exchange.
- J. R. Bateman, A. M. Lee, and C.-t. Wu (2006)
Genetics
173, 769-777
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- Programmed cell death mechanisms of identifiable peptidergic neurons in Drosophila melanogaster.
- Y.-J. Choi, G. Lee, and J. H. Park (2006)
Development
133, 2223-2232
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- Drosophila melanogaster auxilin regulates the internalization of Delta to control activity of the Notch signaling pathway.
- E. J. Hagedorn, J. L. Bayraktar, V. R. Kandachar, T. Bai, D. M. Englert, and H. C. Chang (2006)
J. Cell Biol.
173, 443-452
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- Molecular Genetics of Natural Populations.
- A. J. Greenberg and C.-I Wu (2006)
Mol. Biol. Evol.
23, 883-886
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- The Mcp Element Mediates Stable Long-Range Chromosome-Chromosome Interactions in Drosophila.
- J. Vazquez, M. Muller, V. Pirrotta, and J. W. Sedat (2006)
Mol. Biol. Cell
17, 2158-2165
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