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Science 1 October 1982:
Vol. 218. no. 4567, pp. 66 - 68
DOI: 10.1126/science.7123220
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Articles

Science, Vol 218, Issue 4567, 66-68
Copyright © 1982 by American Association for the Advancement of Science

articles

Development of mouse embryos in vitro: preimplantation to the limb bud stage

LT Chen and YC Hsu

Mouse embryos were grown successfully in vitro from the blastocyst stage to the limb bud stage. Mouse blastocysts grown in vitro for 10 days showed blood circulation in the yilk sac, forelimb buds, and the primordia of liver, pancreas, and lungs. These characteristics are indicative of a developmental stage equivalent to one-half of the total gestation period in utero. Improvements in culture conditions from days 7 to 9 have made it feasible to culture mouse blastocysts beyond the early somite stage.


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P. Pallares, R. A Garcia-Fernandez, L. M Criado, C. A Letelier, D. Esteban, J. M Fernandez-Toro, J. M Flores, and A. Gonzalez-Bulnes (2008)
Reproduction 136, 573-579
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Intrauterine Growth Retardation in Endothelial Nitric Oxide Synthase-Deficient Mice Is Established from Early Stages of Pregnancy.
P. Pallares and A. Gonzalez-Bulnes (2008)
Biol Reprod 78, 1002-1006
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Nitric oxide modulates murine yolk sac vasculogenesis and rescues glucose induced vasculopathy.
A. K. Nath, J. Enciso, M. Kuniyasu, X.-Y. Hao, J. A. Madri, and E. Pinter (2004)
Development 131, 2485-2496
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